Study on chemical constituents and in vitro anti-inflammatory activity of ethyl acetate extraction part from ethanol extract of the roots of Ardisia virens
- VernacularTitle:长叶纽子果根醇提物乙酸乙酯萃取部位的化学成分及体外抗炎活性研究
- Author:
Yongqiang ZHOU
1
;
Zhangrong LIAO
1
;
Hongbo YE
1
;
Xin YIN
1
;
Xin WEI
1
;
Ying ZHOU
1
Author Information
1. College of Pharmacy，Guizhou University of Traditional Chinese Medicine，Guiyang 550025，China
- Publication Type:Journal Article
- Keywords:
roots of Ardisia virens;
ethanol extract;
ethyl acetate extraction part;
chemical constituents;
anti-inflammatory activity
- From:
China Pharmacy
2022;33(17):2072-2076
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To isolate and ide ntify the chemical constituents of the root of Ardisia virens and preliminarily evaluate the in vitro anti-inflammatory activity of the compounds. METHODS The ethyl acetate extraction part from 70％ ethanol extract of the root of A. virens were separated and purified by silica gel column chromatography ，ODS column chromatography ， etc. The structures of the compounds were identified according to physical and chemical properties and spectral data. The inflammation model of RAW 264.7 cells was induced by lipopolysaccharide ，and anti-inflammatory activity of the compound was investigated by MTT assay. RESULTS A total of 11 compounds were isolated from the ethyl acetate extraction part ，and were identified as cyclamiretin A （1），α-spinasterol （2），（3S，5R，6S，7E）-3，5，6-trihydroxy-7-megastigmen-9-one （3）， （+）-angelicoidenol（4），octadeca-dienoic acid- 2，3-dihydroxypropyl ester （5），α-linolenic acid （6），glycerol monooleate （7），5， 5′-（4，7-hexadecadlene-1，16-diyl）bisresorcinol（8），1-（3，5-dihydroxyphenyl）heptan-1-one（9），5-heptylresorcinol and （10） 5-n-nonylresorcinol（11）. The in vitro anti-inflammatory results showed that 80，40，20，10，5 μg/mL of compounds 2，8，9 and 10 could reduce the cell survival rate in different degrees. CONCLUSIONS Compounds 1-11 are isolated from this plant for the first time，and compound 8 is a new natural product. Compound 2，8，9 and 10 show certain anti-inflammatory activity in vitro .
