Effect of Buyang Huanwutang on Rehabilitation of Ischemic Stroke by Cell Membrane Solid-phase Chromatography Combined with Network Pharmacology
10.13422/j.cnki.syfjx.20220111
- VernacularTitle:细胞膜固相色谱法联合网络药理学探讨补阳还五汤促进缺血性脑卒中康复的作用
- Author:
Xiang-li YAN
1
;
Ying-ying HE
2
;
Ming BAI
1
;
Er-ping XU
1
Author Information
1. Academy of Traditional Chinese Medicine，Henan University of Chinese Medicine， Zhengzhou 450046，China
2. College of Chinese Medicine，Guangzhou University of Chinese Medicine，Guangzhou 510006，China
- Publication Type:Journal Article
- Keywords:
Buyang Huanwutang;
ischemic stroke;
cell membrane solid-phase chromatography;
network pharmacology
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(2):191-198
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of Buyang Huanwutang （BHD） on rehabilitation of ischemic stroke（IS） by cell membrane solid-phase chromatography and network pharmacology. MethodCell membrane solid-phase chromatography was performed to screen the specific binding components of BHD with hippocampal neurons. Targets of the specific components were retrieved based on PubChem and PharmMapper and those of IS were searched from Online Mendelian Inheritance in Man （OMIM） and GeneCards. Then， the protein-protein interaction （PPI） network was constructed with STRING and Cytoscape 3.7.1， followed by Gene Ontology （GO） term enrichment and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment of the hub genes in the PPI network. Thereby， the mechanism of BHD in promoting IS rehabilitation was clarified. ResultA total of 13 specific components were identified. The hub genes were mainly involved in the biological processes of regulation of cell proliferation， protein phosphorylation， hypoxia response， and angiogenesis， and the pathways of Forkhead box O （FoxO） signaling pathway， adenosine 5'-monophosphate （AMP）-activated protein kinase （AMPK） signaling pathway， nuclear factor kappa B （NF-κB） signaling pathway， and apoptosis pathway. ConclusionBHD may promote the recovery of IS by regulating FoxO， AMPK， NF-κB， and apoptosis pathways.
