Effect of Hedysarum Polysaccharides on Bcl-2/Caspase-3 Signaling Pathway of Schwann Cells Cultured in High Glucose
10.13422/j.cnki.syfjx.20220694
- VernacularTitle:红芪多糖对高糖培养下雪旺细胞Bcl-2/Caspase-3信号通路的影响
- Author:
Yan-xu CHEN
1
;
Zhi-sheng JIN
1
;
Liu HE
2
;
Chang-qing XU
1
;
Cai-yun JIN
1
;
Lei ZHANG
1
;
Xiao-xue JIANG
1
;
Peng-fei HUAN
3
Author Information
1. Gansu University of Chinese Medicine，Lanzhou 730000，China
2. The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine（TCM）， Guiyang 550000，China
3. Shanghai University of TCM，Shanghai 200000，China
- Publication Type:Journal Article
- Keywords:
Hedysarum polysaccharides;
diabetic peripheral neuropathy;
Schwann cells;
apoptotic response;
B-cell lymphoma 2 （Bcl-2）;
cysteinyl aspartate-specific protease 3 （Caspase-3）;
Bcl-2-associated X protein （Bax）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(6):71-78
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effects of Hedysarum polysaccharides（HPS）on the signaling pathways of B-cell lymphoma 2 （Bcl-2）， cysteinyl aspartate-specific protease 3 （Caspase-3）， and Bcl-2-associated X protein （Bax） in Schwann cells（SCs）cultured in high glucose，and explore the possible mechanism of HPS against diabetic peripheral neuropathy（DPN）. MethodFour SD suckling mice aged 5-7 days were randomly divided into a normal group，a high-glucose group，an HPS + high-glucose group，and an α-lipoic acid（α-LA）+ high-glucose group. SCs were extracted from the sciatic nerve and cultured in a 37 ℃，5% CO2 incubator. After the cells reached 80% confluence，Cell Counting Kit-8（CCK-8）was used to screen the experimental concentrations suitable for high glucose，HPS， and α-LA interventions. Western blot and Real-time polymerase chain reaction （Real-time PCR）were used to detect the protein and mRNA expression of Bcl-2，Bax，and Caspase-3. The apoptosis rate of SCs was detected by flow cytometry using Annexin V-fluorescein isothiocyanate （FITC）/propidium iodide （PI）. ResultAs revealed by Western blot and real-time PCR，compared with the normal group，the high-glucose group showed reduced protein and mRNA expression of Bcl-2 and increased protein and mRNA expression of Bax and Caspase-3（P<0.01）. Compared with the high-glucose group，the HPS + high-glucose group and the α-LA + high-glucose group showed increased protein and mRNA expression of Bcl-2 and decreased protein and mRNA expression of Bax and Caspase-3（P<0.01）. As displayed by the results of flow cytometry using Annexin V/PI， compared with the normal group，the high-glucose group showed increased apoptosis rate；compared with the high-glucose group，the HPS + high-glucose group and the α-LA + high-glucose group showed reduced apoptosis rate（P<0.01）. ConclusionHPS can alleviate the apoptotic response of SCs，and its mechanism may be related to the inhibition of the activation of the Bcl-2/Caspase-3 signaling pathway.
