Mechanism of Xianlian Jiedu Prescription in Maintaining Cancer Relative Vascular Endothelial Cell Homeostasis and Inhibiting Tumor Neovascularization
10.13422/j.cnki.syfjx.20220828
- VernacularTitle:仙连解毒方维持肿瘤相关血管内皮细胞稳态抑制肿瘤血管新生的作用机制
- Author:
Qi-juan WANG
1
;
Wei-xing SHEN
1
;
Rui-yang JIANG
1
;
Chang-liang XU
1
;
Zheng-jie SHEN
2
;
Qiu-ying YAN
3
;
Dong-dong SUN
3
;
Hai-bo CHENG
1
Author Information
1. The First Clinical Medical College of Nanjing University of Chinese Medicine， Nanjing 210023， China
2. Zhangjiagang Hospital Affiliated to Nanjing University of Chinese Medicine， Suzhou 215600， China
3. Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine for Prevention and Treatment of Tumor， Nanjing 210023， China
- Publication Type:Journal Article
- Keywords:
Xianlian Jiedu prescription（XLJDP）;
colorectal cancer;
vascular homeostasis;
angiopoietin2;
conditioned medium
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(8):86-92
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of Xianlian Jiedu prescription （XLJDP） on the proliferation， apoptosis， and migration of cancer-relative endothelial （CRE） cells， and to decipher the mechanism of XLJDP in regulating angiopoietin2 （Ang2） to maintain CRE cell homeostasis and inhibit tumor neovascularization. MethodHuman umbilical vein endothelial cell line （HUVEC-c） was induced into CRE cells in the human colorectal cancer HCT-116 cell-conditioned medium. The CRE cells were assigned into the blank group， conditioned medium group， and XLJDP groups （1， 2， 3 g·L-1） and treated for 48 h. The proliferation of CRE cells was detected by methyl thiazolyl tetrazolium （MTT） colorimetry. The morphological changes of CRE cells were observed via an inverted microscope. The apoptosis rate was detected by flow cytometry. Wound healing test and Transwell migration assay were employed to detect the 2D/3D migration ability of CRE cells. The protein levels of vimentin， N-cadherin， matrix metalloproteinase-9 （MMP-9）， and Ang2 in CRE cells were measured by Western blot. ResultThe MTT results showed that the cell viability was higher in the conditioned medium group than in the blank group （P<0.05）. Compared with the conditioned medium group， XLJDP decreased the cell proliferation rate （P<0.01） and changed the cell morphology. The total apoptosis rates of all the XLJDP groups were higher than that of the conditioned medium group （P<0.01）. The 2D and 3D migration abilities of the conditioned medium group were higher than those of the blank group （P<0.05， P<0.01）. Compared with the conditioned medium group， XLJDP at all the concentrations weakened the 2D migration ability （P<0.01） and medium- and high-concentration XLJDP weakened the 3D migration ability （P<0.01）. The protein levels of N-cadherin， Vimentin， MMP-9， and Ang2 were up-regulated in the conditioned medium group compared with those in the blank group （P<0.05， P<0.01）. Compared with the conditioned medium group， XLJDP at all the concentrations down-regulated the protein level of Ang2 （P<0.05， P<0.01）， and medium- and high-concentration XLJDP down-regulated those of N-cadherin， vimentin， and MMP-9 protein （P<0.01）. ConclusionXLJDP may inhibit the proliferation， migration， differentiation， and apoptosis of CRE cells by down-regulating the expression of Ang2， inhibiting tumor neovascularization， and maintaining the cell homeostasis.
