Isolation and Identification of Chemical Constituents in Seeds of Sophora tonkinensis
10.13422/j.cnki.syfjx.20211760
- VernacularTitle:越南槐种子化学成分的分离与鉴定
- Author:
Ji-bin ZOU
1
;
Peng ZHANG
2
;
Qiao AN
2
;
Yang-ming JIANG
2
;
Yu XIA
2
;
Zhan-xing HU
2
;
Xiao-jiang HAO
1
Author Information
1. School of Pharmaceutical Sciences，Guizhou Medical University，Guiyang 550025，China
2. Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences，Guiyang 550014，China
- Publication Type:Journal Article
- Keywords:
Sophora;
seeds of S. tonkinensis;
chemical constituents;
flavonoids;
isoflavonoids;
terpenoids;
Helicobacter pylori
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(1):165-172
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the chemical constituents of the seeds of Sophora tonkinensis. MethodThe chemical constituents were isolated and purified by chromatography with MCI resin， silica gel， Sephadex LH-20， and semi-preparative high performance liquid chromatography. Their structures were identified by physicochemical properties， spectral data as well as relevant references. Meanwhile， the antibacterial activities against Helicobacter pylori of these compounds were screened by agar dilution method. ResultA total of 22 compounds were isolated from the methanol extract of the seeds of S. tonkinensis， and characterized as 4′，7-dihydroxy-6-methoxy isoflavone （1）， daidzein （2）， wighteone （3）， dalparvone （4）， 5，7-dihydroxy-4′-methoxyisoflavone （5）， prunetin （6）， formononetin （7）， genistein （8）， 5-methoxydaidzein （9）， ononin （10）， 7，4′-dihydroxyflavone （11）， liquiritigenin （12）， bayin （13）， 2，4-dihydroxybenzoate （14）， methyparaben （15）， 4-hydroxyacetophenone （16）， p-anisaldehyde （17）， methyl indole-3-carboxylate （18）， 4-［β-D-apiofuranoyl-（1→6）-O-β-D-glucopyranosyloxy］ phenylacetonitrile （19）， （-）-methyl dihydrophaseate （20）， methyl canavaliol ester （21）， vomifoliol 3′-O-β-D-apiofuranosyl-（1→6）-β-D-glucopyranoside （22）. ConclusionCompounds 1， 5， 6， 9 and 16 are isolated from S. tonkinensis for the first time， compounds 4， 14， 17-22 are isolated from the genus of Sophora for the first time. In addition， compounds 10 and 13 display moderate antibacterial activities against H. pylori.
