Effect of Danggui Niantongtang on Expression of Autophagy-related Proteins LC3， Beclin1 and p62 in Rats with Adjuvant Arthritis Differentiated into Wind-damp-heat Impediment
10.13422/j.cnki.syfjx.20211842
- VernacularTitle:当归拈痛汤对风湿热痹型佐剂性关节炎大鼠自噬蛋白LC3，Beclin1，p62表达的影响
- Author:
Qi-jin LU
1
;
Jia-yu LI
1
;
Yi-si CAI
1
;
Fan ZHAO
2
;
Hong-xin LIN
1
;
Gang LIU
3
;
Li-xia YUAN
1
Author Information
1. School of Traditional Chinese Medicine，Southern Medical University，Guangzhou 510515，China
2. Shenzhen Hospital of Guangzhou University of Chinese Medicine （Futian），Shenzhen 518034， China
3. Nanfang Hospital of Southern Medical University， Guangzhou 510515， China
- Publication Type:Journal Article
- Keywords:
Danggui Niantongtang;
wind-damp-heat impediment;
rheumatoid arthritis;
microtubule-associated protein 1 light chain 3 （LC3）;
homolog of yeast Atg6 （Beclin1）;
p62;
autophagy
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2022;28(1):41-49
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the preventive and control effects of Danggui Niantongtang against adjuvant arthritis differentiated into wind-damp-heat impediment in rats and its influences on the expression of autophagy-related proteins microtubule-associated protein 1 light chain 3 （LC3）， homolog of yeast Atg6 （Beclin1） and p62. MethodThe six-week-old male SD rats were randomly divided into the normal group， wind-damp-heat impediment model group， low-， medium-， and high-dose Danggui Niantongtang （5.67， 11.34， 22.68 g·kg-1） groups， and methotrexate （MTX， 1.35 mg·kg-1） group， with 10 rats in each group. A rat model of adjuvant arthritis was established by subcutaneous injection of inactivated Mycobacterium tuberculosis into the tail root， followed by exposure to the manual climatic box for 16 d for inducing the wind-damp-heat impediment. The drugs were administered intragastrically on the day of immunization for 28 d. The general conditions of rats were observed and the swelling degree of toes and arthritis index （AI） were detected. The pathological changes in the synovial tissues of the knee joints were observed by hematoxylin-eosin （HE） staining. The mRNA expression levels of LC3， Beclin1， and p62 in the synovial tissues were measured by real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）， followed by the assay of their protein expression by Western blot and immunohistochemistry. ResultCompared with the normal group， the wind-damp-heat impediment model group exhibited significantly increased swelling degree of toes （P<0.01）， increased AI （P<0.01）， proliferated synovial cells （P<0.01）， up-regulated LC3 and Beclin1 protein and mRNA expression （P<0.01）， and down-regulated p62 protein and mRNA expression （P<0.01） after 16， 20， 24， 28-d medication. Compared with the wind-damp-heat impediment model group， each medication group displayed alleviated toe swelling and synovial hyperplasia to different degrees， decreased mRNA and protein expression levels of LC3 and Beclin1 （P<0.01）， and increased p62 mRNA and protein expression （P<0.05，P<0.01）， with the best outcomes observed in the medium-dose Danggui Niantongtang group. ConclusionDanggui Niantongtang effectively relieves adjuvant arthritis due to wind-damp-heat impediment in rats， which may be related to its regulation of the expression of autophagy-related proteins LC3， Beclin1， and p62 and the inhibition of autophagy.
