Expression of inflammatory factors in peripheral blood of patients with HLA-B27 positive and negative uveitis
10.3980/j.issn.1672-5123.2022.9.28
- VernacularTitle:HLA-B27阳性与阴性葡萄膜炎患者外周血中炎症因子表达研究
- Author:
Hong-Ya WANG
1
;
Wen ZHENG
1
;
Yong-Gang DAI
1
;
Wei-Yan ZHOU
1
;
Jin-Xue SUN
1
Author Information
1. Department of Clinical Laboratory, Shandong Provincial Third Hospital, Jinan 250031, Shandong Province, China
- Publication Type:Journal Article
- Keywords:
uveitis;
human leukocyte antigen-27(HLA-B27);
inflammatory factor
- From:
International Eye Science
2022;22(9):1559-1563
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the correlation by analyzing and comparing the expression of inflammatory factors in peripheral blood of human leukocyte antigen-B27(HLA-B27)positive and negative uveitis patients.METHODS: All 76 patients first diagnosed with uveitis in our hospital from January 2020 to April 2022 were screened in this retrospective study. Nucleated cells were isolated from human venous blood, and HLA-B27 was detected by flow cytometry(direct immunofluorescence), the patients were divided into the HLA-B27-positive group(≥90%)in 35 cases and HLA-B27-negative group(≤5%)in 41 cases. The whole blood RNA was extracted. The mRNA expression of interleukin-1β(IL-1β), interleukin-6(IL-6), interleukin-10(IL-10), protease activated receptor 2(PAR2), tumor necrosis factor-α(TNF-α), interleukin enhancer-binding factor -3(ILF3)were detected and compared by RT-qPCR.RESULTS: The IL-1β, IL-10, PAR2 and TNF-α mRNA were observed no difference between the two groups of patients(all P>0.05). The IL-6 mRNA in the patients of HLA-B27-positive group was higher than in the HLA-B27-negative group, the ILF3 mRNA was lower than that in the negative group(all P<0.05).CONCLUSION: The expression level of IL-6 in peripheral blood was significantly increased and ILF3 was decreased in HLA-B27-positive group, which can be used as auxiliary indicators for diagnosis and treatment of HLA-B27-positive uveitis.
