The Effect of Matrix Remodeling Associated 7 (MXRA7) Expression on the Biological Function of SHI-1 Cells.
10.19746/j.cnki.issn.1009-2137.2022.03.005
- Author:
Yu-Dan ZHENG
1
;
Zheng-Jiang SUN
1
;
Kun-Peng MA
1
;
Yi-Qiang WANG
1
;
Dan-Dan LIN
2
,
3
Author Information
1. Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Medical College of Soochow University, Suzhou 215000, Jiangsu Province, China.
2. Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Medical College of Soochow University, Suzhou 215000, Jiangsu Province, China,E-mail: szlindd@
3. com.
- Publication Type:Journal Article
- Keywords:
BCL-2;
MXRA7;
acute myeloid leukemia;
cell apoptosis
- MeSH:
Apoptosis;
Apoptosis Regulatory Proteins;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Humans;
Membrane Proteins/metabolism*;
Proto-Oncogene Proteins c-bcl-2/metabolism*
- From:
Journal of Experimental Hematology
2022;30(3):688-694
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To express matrix remodeling associated 7 (MXRA7) in the human acute myeloid leukemia SHI-1 cell line and to assess the role of MXRA7 in the biological function of SHI-1 cells.
METHODS:The full-length cDNA sequence of human MXRA7 was synthesized and subcloned into the lentivirus shuttle vector pRRL-Venus. SHI-1 cells were transfected with the lentivirus which was packaged with 293T cells. The YFP-positive cells were sorted by flow cytometry and the stable cell lines were obtained by expanded culture. The expression and distribution of MXRA7 in SHI-1 cells were verified by real-time qPCR, Western blot and laser confocal techniques. Cell proliferation and cell cycle were measured by flow cytometry, and apoptosis was determined by Annexin V and 7-AAD staining. The expression of apoptosis related proteins were detected by Western blot.
RESULTS:The stable SHI-1 cell line overexpressing MXRA7 was established successfully. Laser confocal analysis confirmed that MXRA7 was expressed in the cytoplasm of SHI-1 cells. Compared with the control cell line, the overexpression of MXRA7 showed no effect on the cell proliferation and cell cycle, but reduced the percentage of apoptosis cells induced by methotrexate. Moreover, the expression of BCL-2 protein was increased by overexpression of MXRA7, which can inhibit cell apoptosis.
CONCLUSION:The SHI-1 stable cell line overexpressing MXRA7 was established successfully, and MXRA7 could inhibit drug-induced apoptosis through increasing the expression of BCL-2 protein.
