Establishment of Neurotoxicity Assessment Using Microelectrode Array (MEA) with hiPSC-Derived Neurons and Evaluation of New Psychoactive Substances (NPS)
- Author:
Kyu-ree KANG
1
;
C-Yoon KIM
;
Jin KIM
;
Bokyeong RYU
;
Seul-Gi LEE
;
Jieun BAEK
;
Ye-Ji KIM
;
Jin-Moo LEE
;
Yootmo LEE
;
Sun-Ok CHOI
;
Dong Ho WOO
;
Il Hwan PARK
;
Hyung Min CHUNG
Author Information
- Publication Type:ORIGINAL ARTICLE
- From:International Journal of Stem Cells 2022;15(3):258-269
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background and Objectives:Currently, safety pharmacological tests for the central nervous system depend on animal behavioral analysis. However, due to the subjectivity of behavioral analysis and differences between species, there is a limit to appropriate nervous system toxicity assessment, therefore a new neurotoxicity assessment that can simulate the human central nervous system is required.
Methods:and Results: In our study, we developed an in vitro neurotoxicity assessment focusing on neuronal function. To minimize the differences between species and fast screening, hiPSC-derived neurons and a microelectrode array (MEA) that could simultaneously measure the action potentials of the neuronal networks were used. After analyzing the molecular and electrophysiological characters of our neuronal network, we conducted a neurotoxicity assessment on neurotransmitters, neurotoxicants, illicit drugs, and new psychoactive substances (NPS). We found that most substances used in our experiments responded more sensitively to our MEA-based neurotoxicity assessment than to the conventional neurotoxicity assessment. Also, this is the first paper that evaluates various illicit drugs and NPS using MEA-based neurotoxicity assessment using hiPSC-derived neurons.
Conclusions:Our study expanded the scope of application of neurotoxicity assessment using hiPSC-derived neurons to NPS, and accumulated evaluation data of various toxic substances for hiPSC-derived neurons.
