Identification of chemical constituents in Shenfu injection and study on anti-inflammatory activities of its poly- acetylene compounds
- VernacularTitle:参附注射液化学成分鉴定及其炔醇类化合物的抗炎活性研究
- Author:
Qi ZHENG
1
;
Xiaoya WANG
1
;
Jie LIU
1
;
Cheng PENG
2
;
Liang XIONG
1
;
Yaning ZHU
3
;
Qinmei ZHOU
1
Author Information
1. State Key Laboratory of Southwestern Chinese Medicine Resources/School of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China;Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
2. State Key Laboratory of Southwestern Chinese Medicine Resources/School of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
3. China Resources Sanjiu （Ya’an） Pharmaceutical Co.，Ltd.，Sichuan Ya’an 625000，China
- Publication Type:Journal Article
- Keywords:
Shenfu injection;
polyacetylenes;
anti-
- From:
China Pharmacy
2022;33(16):1931-1936
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the chemical constituents in S henfu injection and the anti-inflammatory activities of its polyacetylene compounds. METHODS Shenfu injection was separated and purified by macroporous adsorption resin ，medium pressure liquid chromatography ，preparative thin layer chromatography and reversed-phase semi-preparative high-performance liquid chromatography，and the compound structure was identified according to the physicochemical properties and spectral data. RAW 264.7 cell inflammation model was used to evaluate the anti-inflammatory activities of polyacetylene compounds . The effects of active polyacetylene compounds on the expressions of cyclooxygenase- 2（COX-2）protein were evaluated by Western blot assay. RESULTS Twelves compounds were isolated and identified from Shenfu injection ，including 8 ginsenoside compounds ，i.e. ginsenoside Rg 1（1），ginsenoside Re （2），ginsenoside Rb 1（3），ginsenoside Rk 1（4），20（R）-ginsenoside Rh 1（5），20（S）-ginsenoside Rg3 （6），notoginsenoside R 1（7），panaxatriol（8）；4 polyacetylene compounds ，i.e.（3R，9R，10R）-panaxytriol（9），panaxydol（10）， heptadeca-1，8-dien-4，6-diyne-3，10-diol（11）and panaxynol （12）. Among 4 polyacetylene compounds ，only compound 10 had anti-inflammatory activity. Compound 10 was not toxic to normal RAW 264.7 cells；when the concentration of compound 10 ranged 12.5-50.0 μmol/L，it could significantly reverse the lipopolysaccharide-induced NO content increase in cell supernatant （P＜0.05 or P＜0.01）；when the concentration of co mpound 10 was 50.0 μmol/L，it could significantly reverse the lipopolysaccharide-induced protein expression increase of COX- 2 in cells （P＜0.05）. CONCLUSIONS Compounds 4，7，10-12 are identified and reported in Shenfu injection for the first time ，and panaxydol possesses a certain anti-inflammatory effect.
