Study on intermittent fasting changing fecal metabolism pattern of mice based on metabolomics technique
10.16438/j.0513-4870.2022-0309
- VernacularTitle:基于代谢组学技术探讨间歇性禁食对小鼠粪便代谢物的影响
- Author:
Xing XIONG
1
;
Wei XIONG
2
;
Yan CHEN
3
;
Zheng-cai JU
3
Author Information
1. School of Pharmacy, Nantong University, Nantong 226000, China
2. College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350000, China
3. Teaching Experiment Center of Shanghai University of Traditional Chinese Medicine, Shanghai 200120, China
- Publication Type:Research Article
- Keywords:
intermittent fasting;
metabolomics;
fecal;
metabolite;
pathway analysis
- From:
Acta Pharmaceutica Sinica
2022;57(7):2183-2190
- CountryChina
- Language:Chinese
-
Abstract:
Increasingly researches suggested that intermittent fasting (IF) can be part of a healthy lifestyle, which can improve risk factors associated with obesity and diabetes. Based on LC-MS metabolomics technology, this study preliminarily discussed the effect of IF on the metabolism of mice under normal physiological conditions by detecting the fecal metabolites. All experiments were approved by the Animal Research Committee of Shanghai University of Traditional Chinese Medicine. Animal welfare and the animal experimental protocols were strictly consistent with related ethics regulations of Shanghai University of Traditional Chinese Medicine. The mice were subjected to intermittent fasting for 10 weeks by fasting for 24 hours on alternate days, and their feces at 5 and 10 weeks were collected. The results show that the weight, food and water intake was not significant altered in mice with IF, but fasting blood glucose level was decreased. We found that fecal metabolites change was present at 5-week, being more prominent in 10-week. Relative to the control mice, we detected 17 and 108 metabolites in two time points, respectively. These metabolites were mainly enriched in the pathways of linoleic acid metabolism and bile acid biosynthesis. We further found that 10 metabolites may be a close correlation with IF, which had the same change trend at two time points. In conclusion, the present study provides a new approach to study the metabolism mechanism in IF treatment of related diseases.
