Interleukin-17A is closely correlated with the progression of renal epithelial-mesenchymal transition in spontaneously hypertensive rats.
10.12122/j.issn.1673-4254.2022.05.21
- Author:
Mao Cui XIANG
1
;
Yu WANG
1
;
Ren Biao MEI
1
;
Ji Feng FU
1
;
Jing CHEN
1
;
Chang Le DU
1
Author Information
1. Department of Medical Functional Sciences, Medical College of Anhui University of Science & Technology, Huainan 232001, China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
epithelial-mesenchymal transition;
high blood pressure;
interleukin-17A;
macrophages
- MeSH:
Animals;
Blood Pressure;
Cadherins/metabolism*;
Epithelial-Mesenchymal Transition;
Hypertension;
Interleukin-17/metabolism*;
Kidney;
RNA, Messenger/metabolism*;
Rats;
Rats, Inbred SHR;
Rats, Inbred WKY
- From:
Journal of Southern Medical University
2022;42(5):772-779
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the role of interleukin-17A (IL-17A) in renal epithelial- mesenchymal transition (EMT) in essential hypertensive nephropathy.
METHODS:Four-week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats (control group) were both randomized into 4 groups (n=5) for observation at 4, 6, 10 and 30 weeks of age. Blood pressure of the rats was monitored using a noninvasive tail artery blood pressure measurement instrument. The percentage of Th17 cells in the splenocytes was analyzed using flow cytometry. The mRNA and protein expression levels of IL-17A, iNOS, Arg-1, E-cadherin, and α-SMA in the kidneys of the rats were detected using RT-PCR and immunohistochemical staining, respectively, and plasma levels of IL-17A were regularly detected using ELISA.
RESULTS:At the age of 6 weeks, the SHRs began to show significantly higher blood pressure with greater Th17 cell percentage in the splenocytes and high renal expression and plasma level of IL-17A than WKY rats (P < 0.05 or P < 0.01). At 30 weeks, renal expression of E-cadherin mRNA and protein was significantly lower and the expression of Arg-1 mRNA and protein was significantly higher in SHR than in WKY rats (P < 0.01). Compared with the WKY rats, the SHRs showed significantly higher mRNA and protein expressions of iNOS at 6 and 10 weeks (P < 0.05 or 0.01) and higher α-SMA mRNA and protein expressions since 10 weeks of age (P < 0.05 or 0.01). In SHRs older than 10 weeks, renal IL-17A mRNA and protein expression levels were negatively correlated with those of E-cadherin (r=-0.731, P < 0.05; r=-0.827, P < 0.01) and positively correlated with those of α-SMA (r=0.658, P < 0.05; r=0.968, P < 0.01).
CONCLUSION:IL-17A is closely correlated with the progression of renal EMT in SHR and plays its role possibly by mediating M1/M2 polarization of renal infiltrating macrophages.
