Culture of pluripotent stem cell-derived hepatocytes and organoids.
10.3760/cma.j.cn501113-20220318-00120
- VernacularTitle:多能干细胞源性肝细胞和类器官的培养
- Author:
Jie Ying ZHU
1
;
Duan Qing PEI
2
Author Information
1. Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510535, China.
2. West Lake University, Hangzhou 310024, China.
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Hepatocytes;
Humans;
Induced Pluripotent Stem Cells;
Liver;
Organoids;
Pluripotent Stem Cells
- From:
Chinese Journal of Hepatology
2022;30(3):244-248
- CountryChina
- Language:Chinese
-
Abstract:
Liver is one of the most important organs in the human body. Liver diseases are also a major threat to human health and longevity. Hepatic decompensation treatment is quite difficult due to multiple reasons. Extracorporeal liver support devices are unable to solve this problem, and there is a severe shortage of orthotopic liver transplant donors. Study of pluripotent stem cell-derived hepatocytes and organoids can determine not only hepatocyte fate, but also liver development, regeneration mechanisms, and pathophysiology. Furthermore, it can be used for drug screening in order to provide a stable source of functional hepatocytes for future transplantation therapy. Culture of pluripotent stem cell-derived hepatocytes and organoids has a self-organizing process similar to liver development, i.e., starting with changes in several key factors, and eventually forming functionally complex cells/organs. This paper introduces the main methods and progress of pluripotent stem cell-derived hepatocytes and organoids, with hope to provide clues for future research.
