The IL-6/STAT3 signaling pathway mediated by radiotherapy regulates the expression of PD-L1 in esophageal cancer cells.
10.3760/cma.j.cn112152-20200320-00236
- VernacularTitle:放疗介导白细胞介素6/信号转导和转录激活因子3信号通路调节食管癌细胞中程序性死亡蛋白配体1的表达
- Author:
Yi Chen ZHOU
1
;
Xue Zhou PANG
1
;
Hong Lei ZHU
1
;
Yu HE
1
;
Yang SHEN
1
;
Dai Yuan MA
1
Author Information
1. Department of Oncology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China.
- Publication Type:Journal Article
- Keywords:
Esophageal carcinoma;
IL-6/STAT3 pathway;
Programmed cell death ligand-1;
Radiotherapy
- MeSH:
B7-H1 Antigen/metabolism*;
Cell Line, Tumor;
Cell Proliferation;
Esophageal Neoplasms/radiotherapy*;
Humans;
Interleukin-6/metabolism*;
RNA, Messenger;
STAT3 Transcription Factor/metabolism*;
Signal Transduction
- From:
Chinese Journal of Oncology
2022;44(5):389-394
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the possible mechanism of radiotherapy regulating the expression of PD-L1 in esophageal carcinoma. Methods: Three esophageal cancer cell lines (Eca109, Kyse150, TE1) were irradiated with different doses of X-rays, and 6 Gy+ AG490 group was set. The mRNA expression of PD-L1 was detected by real-time quantitative polymerase chain reaction (RT-qPCR). The protein expressions of PD-L1, STAT3, p-STAT3 were detected by western blotting and the protein level of IL-6 was detected by ELISA. Results: The mRNA expressions of PD-L1 in Eca109, Kyse150 and TE1 were 2.86±0.30, 960.01±21.27 and 106.78±6.67, higher than 1.07±0.15 in normal esophageal cell line HET-1A (P<0.01). The protein expressions of PD-L1 in Eca109, Kyse150 and TE1 were 0.091±0.036, 1.533±0.079 and 0.914±0.035, higher than 0.063±0.01 in normal esophageal cell line HET-1A (P<0.01). After 48 hours of 6 Gy irradiation, the protein expression levels of PD-L1 in Eca109, Kyse150 and TE1 were 0.135±0.007, 1.66±0.06 and 1.32±0.06, higher than 0.09±0.01, 1.21±0.05 and 0.93±0.03 of the 0 Gy group (P<0.01), while the protein expression levels of p-STAT3 in Eca109, Kyse150 and TE1 were 1.44±0.26, 0.75±0.04 and 1.92±0.17, higher than 0.18±0.05, 0.48±0.02 and 0.36±0.06 of the 0 Gy group (P<0.01). IL-6 protein expression increased significantly after different doses of irradiation (P<0.01). After the IL-6/STAT3 signaling pathway was blocked by the specific inhibitor AG490, the expressions of PD-L1 of Eca109, Kyse150 and TE1 in the 6 Gy+ AG490 groups were 0.11±0.03, 1.07±0.08 and 0.96±0.11, without significant differences of 0.09±0.01, 0.96±0.05 and 0.85±0.09 of the 0 Gy group (P>0.05), while the protein expressions of p-STAT3 were 0.76±0.11, 0.59±0.06 and 0.96±0.12, without significant differences of 0.67±0.08, 0.54±0.06 and 0.84±0.11 of the 0 Gy group (P>0.05). Conclusion: Radiotherapy may regulate the expression of PD-L1 in esophageal cancer cells through IL-6 / STAT3 signaling pathway.
