Study on the apoptosis of human retinal pigment epithelium cells induced by EGCG
10.3980/j.issn.1672-5123.2022.8.04
- VernacularTitle:EGCG诱导人视网膜色素上皮细胞凋亡作用的研究
- Author:
Mei-Yuan QIU
1
;
Zhi-Xiang DING
1
;
He JIN
1
;
Jiao-Jiao JIANG
1
Author Information
1. Department of Ophthalmology, the Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China
- Publication Type:Journal Article
- Keywords:
epigallocatech gallate(EGCG);
human retinal pigment epithelium cells(ARPE-19);
apoptosis;
B lymphocytoma-2 gene(bcl-2);
BCL2-Associated X protein(Bax);
caspase-3;
p53
- From:
International Eye Science
2022;22(8):1257-1261
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of epigallocatechin gallate(EGCG)on the apoptosis of human retinal pigment epithelium(ARPE-19)cells and its mechanism. METHODS:The ARPE-19 cells were cultured in vitro and treated with 0,40,80 and 160 μg/mL EGCG, respectively. At the proposed time of treatment the morphological changes were detected by hoechst 33258 staining. The apoptosis rate was detected by flow cytometry. The expression of apoptosis-related factors B lymphocytoma-2 gene(bcl-2), BCL2-Associated X protein(Bax),caspase-3 and p53 were detected by quantitative RT-PCR and Western blotting.RESULTS: Hoechst 33258 staining showed that the ARPE-19 cells with the increase of EGCG drug concentration, the number of apoptotic cells gradually increased and the apoptotic bodies were observed. Flow cytometry showed that the apoptosis rate increased gradually with the increase of EGCG drug concentration. The apoptosis rates at 40, 80 and 160 μg/mL were 4.95%±0.071%, 11.75%±0.075% and 21.25%±0.919% respectively, which was significantly different compared with the control group(2.8%±1.556%)(P<0.01), presented with a drug concentration-dependent. The results of quantitative PCR and Western blotting showed that EGCG could significantly up-regulate the expression of apoptosis-promoting factors Bax, caspase-3 and the mRNA and protein expression of p53, and down-regulate the apoptosis-inhibiting factor bcl-2, all of these showed concentration-dependent effects.CONCLUSION:EGCG can obviously induce the apoptosis of ARPE-19 cells. The mechanism is related with the inhibition of bcl-2 and increase the expression of Bax, caspase-3 and p53.
