Clinical application of multiplex nucleic acid detection technologies: advancements and future
10.3760/cma.j.cn114452-20220223-00087
- VernacularTitle:多重核酸检测技术的临床应用现状及未来
- Author:
Jinchao LI
1
;
Bin XIAO
;
Junfeng XUAN
;
Zhaohui SUN
;
Linhai LI
Author Information
1. 广州医科大学附属第六医院 清远市人民医院检验医学部，清远 511500
- Keywords:
Polymerase chain reaction;
Isothermal amplification technology;
Multiplex nucleic acid detection;
CRISPR/Cas;
Metagenomics next generation sequencing
- From:
Chinese Journal of Laboratory Medicine
2022;45(4):428-432
- CountryChina
- Language:Chinese
-
Abstract:
With the advantage of being capable of detecting multiple targets at the same time, high throughput and cost-effective, multiplex nucleic acid detection technologies meet the need of large-scale nucleic acid screening and quantification. Multiplex polymerase chain reaction has been applied to detect pathogen, methylated DNA, mutated gene, and single nucleotide polymorphism typing. Isothermal amplification technologies, such as loop-mediated isothermal amplification and recombinase polymerase amplification are promising in the field of point-of-care testing. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas)-based multiplex nucleic acid detection technologies have become a hotspot due to their high sensitivity and specificity. Metagenomics sequencing plays a leading role in the detection of emerging pathogens and their gene mutation monitoring as well as tumor research. In this review, the advancements and future of multiplex acid detection technologies in clinical application are discussed.
