Principle of recombinase polymerase amplification and its application progress in medical laboratory science
10.3760/cma.j.cn114452-20220217-00080
- VernacularTitle:重组酶聚合酶扩增技术原理及其在医学检验中的应用进展
- Author:
Meiyun SHANG
1
;
Shaoli DENG
;
Weiping LU
;
Qing HUANG
Author Information
1. 陆军军医大学（第三军医大学）大坪医院检验科，重庆 400042
- Keywords:
Laboratory techniques and procedures;
Nucleic acid amplification techniques;
Isothermal amplification;
Recombinase polymerase amplification
- From:
Chinese Journal of Laboratory Medicine
2022;45(4):423-427
- CountryChina
- Language:Chinese
-
Abstract:
Recombinase polymerase amplification (RPA) is a novel technology for nucleic acid isothermal amplification. It can achieve the rapid amplification and detection of a target gene under 37-42 ℃. This amplification method is highly sensitive, more specific and less instrument-dependent than other existing methods, and it can also integrate multiple detection modes. Therefore, it is especially suitable for applying in low-resource settings and conducting point-of-care tests. Starting from the reaction principles and the experimental design of RPA, this article pointed out some key points when using RPA in a clinical setting. The current development and related problems of RPA were concluded and the various future uses of this method were also prospected.
