Screening and identification of H-2 d-restricted T cell epitopes in fusion and attachment glycoproteins of Nipah virus by ELISPOT
10.3760/cma.j.cn112309-20220124-00026
- VernacularTitle:应用ELISPOT方法筛选确定尼帕病毒融合糖蛋白和附着糖蛋白的H-2 d限制性T细胞表位
- Author:
Mengjing HUANG
1
;
Yao DENG
;
Zhimin ZHAO
;
Jinni CHEN
;
Jiao REN
;
Wen WANG
;
Xiaoling SHEN
;
Wenjie TAN
Author Information
1. 内蒙古医科大学基础医学院微生物教研室，呼和浩特 010010
- Keywords:
Nipah virus;
H-2 d restriction;
T cell epitope;
IFN-γ ELISPOT
- From:
Chinese Journal of Microbiology and Immunology
2022;42(4):287-292
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen and identify H-2 d-restricted T cell epitopes in fusion (F) and attachment (G) glycoproteins of Nipah virus (NiV) in mice. Methods:The complete peptides (single peptide contains 15 amino acids, and 10 amino acids were repeated in the front and back peptides) derived from F and G antigens were mixed into peptide libraries. BALB/c mice were immunized with DNA vaccines expressing NiV F and G proteins alone and in combination. The full sequence peptide libraries of F and G antigens were mixed into peptide pools by matrix design, and spleen cells of immunized mice were collected and analyzed by IFN-γ ELISPOT assay to detect the dominant H-2 d-restricted epitope peptides. Results:Twelve dominant H-2 d-restricted peptides were screened from the F protein-specific peptide library and the 56th peptide produced the strongest reaction. Four dominant peptides were screened from the G protein-specific peptide library and the 72nd peptide produced the strongest reaction. Conclusions:In this study, 12 F antigen-specific and 4 G antigen-specific H-2 d restricted dominant T cell epitopes of NiV were screened and identified by IFN-γ ELISPOT, which could provide reference for immunological analysis of NiV and vaccine research.
