p53 gene mutation and numb expression in colorectal cancer and its clinical significance
10.3760/cma.j.cn113855-20210920-00561
- VernacularTitle:p53基因突变和Numb蛋白表达与结直肠癌临床病理学特征及预后的关系
- Author:
Hui ZHANG
1
;
Yingjiang YE
;
Jing ZHOU
Author Information
1. 北京大学人民医院胃肠外科, 北京100044
- Keywords:
Colorectal neoplasms;
Gene, p53;
Mutation;
Pathology, Clinical;
Prognosis
- From:
Chinese Journal of General Surgery
2022;37(2):122-126
- CountryChina
- Language:Chinese
-
Abstract:
Objective:to study the mutation of p53 gene in colorectal cancer, analyze the relationship between p53 gene mutation and numb expression pattern, and explore its clinicopathological significance in colorectal cancer.Methods:p53 gene mutation in 60 colorectal cancer tissues was analyzed by polymerase chain reaction (PCR) and DNA sequencing, and the expression of numb protein was detected by Western blot. The colon cancer cell lines HCT116 (+), HCT116 (-) and flow cytometry were used. The survival curve was drawn by Kaplan Meier method.Results:p53 gene mutation was found in 31 of 60 tissues (52%), and the mutation times of exons (E) 5, 6, 7 and 8 were 5, 6, 12 and 11 respectively. The expression level of numb in p53 mutation group was significantly lower than that in non mutation group ( P=0.009). The prognosis of patients with low expression of numb (39 cases) was worse than that of high expression of numb (21 cases) ( P=0.015). Its expression level is closely related to the degree of differentiation, lymph node metastasis and TNM stage (all P<0.05). After the two cell lines were transferred into numb, the cell cycle appeared G2-M phase arrest and proliferation was inhibited, while dapt had G1-S phase arrest. Conclusion:p53 gene mutation related to the expression of numb in colon cancer, which has significant effect on the prognosis.
