Expression profile and clinical significance of miRNA in children with autism spectrum disorder
10.3760/cma.j.cn431274-20211014-01074
- VernacularTitle:儿童孤独症谱系障碍患者外周血miRNA表达谱及临床意义
- Author:
Jun QIU
1
;
Yanru CHEN
;
Shuaizheng GONG
;
Nanfei HU
;
Li LIU
;
Ye MA
;
Lijuan LIANG
;
Jie TANG
Author Information
1. 湖南省儿童医院儿科医学研究所，长沙 410007
- Keywords:
Autism spectrum disorder;
MicroRNAs;
Microchip analytical procedures
- From:
Journal of Chinese Physician
2022;24(5):700-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The purpose of this study was to analyze the expression profile of miRNAs in children with autism spectrum disorders (ASD), and to discuss the clinical significance of differentially expressed miRNAs.Methods:MiRNA microarray was used to analyze the expression of miRNA in peripheral blood of 3 pairs of ASD patients-healthy controls; 17 pairs of ASD patients-healthy controls were used to verify the differentially expressed miRNA; Receiver operating characteristic (ROC) curve was used to analyze the differential expression the value of miRNA in the diagnosis of ASD.Results:A total of 32 differentially expressed genes were screened by 3 pairs of miRNA microarray including 12 up-regulated miRNAs and 20 down-regulated miRNAs. miRNA verification of 20 differentially expressed miRNAs showed miR-15a-5p, miR-27a-3p , miR-142-3p and miR-142-5p were significantly down-regulated in children with ASD, with statistically significant difference (all P<0.05). ROC curve analysis showed that the area under the curve (AUC) of the above four miRNAs diagnosing ASD were all greater than 0.70, with sensitivities 94.12%, 100%, 100%, and 82.35%, respectively. Conclusions:The expression of miR-142-3p, miR-27a-3p/miR-15a-5p, and miR-142-5p is down-regulated in the peripheral blood of ASD patients, and has the potential as biomarkers for early screening of ASD.
