Effect of CXC chemokine receptor 7 on cell cycle of neuronal cells in ischemic stroke
10.3760/cma.j.cn121430-20211221-01898
- VernacularTitle:CXC趋化因子受体7对缺血性脑卒中神经元细胞周期的影响
- Author:
Xueying WANG
1
;
Haining MENG
;
Songmei WANG
;
Weifeng XIE
;
Yan QU
Author Information
1. 青岛大学医学部，山东青岛 266071
- Keywords:
Ischemic stroke;
Oxygen-glucose deprivation/reoxygenation;
CXC chemokine receptor 7;
Protein kinase B;
Cell cycle
- From:
Chinese Critical Care Medicine
2022;34(2):151-155
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the function and mechanism of CXC chemokine receptor 7 (CXCR7) in neuronal cells of ischemic stroke.Methods:The expression of CXCR7 in human neuroblastoma SH-SY5Y cells was interfered by small interfering RNA (si-RNA) technique. Oxygen-glucose deprivation/reoxygenation (OGD/R) injury model was constructed in SH-SY5Y cells. CXCR7 protein expression and cell cycle were detected by flow cytometry (FCM). The protein expression of CXCR7 and Akt signaling pathway was detected by Western blotting.Results:After 6 hours of OGD/R, the expression of CXCR7 was significantly decreased compared with OGD/R 0 hour (CXCR7/GAPDH: 0.483±0.098 vs. 1.000±0.000 by Western blotting and 0.686±0.0524 vs. 1.000±0.000 by FCM, both P < 0.01), cell cycle arrest in G0/G1 phase (1.190±0.040 vs. 1.000±0.000, P < 0.01). After CXCR7 si-RNA interference with SH-SY5Y cells, OGD/R was constructed again for 6 hours. Compared with negative control group (si-NC group) under the same environment, the expression of CXCR7 and phosphorylated Akt (p-Akt) was significantly decreased (CXCR7/GAPDH: 0.471±0.051 vs. 1.000±0.000, p-Akt/GAPDH: 0.616±0.027 vs. 1.000±0.000, both P < 0.001) and cell cycle arrest in G0/G1 phase (1.105±0.033 vs. 1.000±0.000, P < 0.05). Conclusion:The CXCR7 could regulate the cycle of neuronal cells in ischemic stroke through Akt signaling pathway, which has a protective effect on neuronal cells.
