Optimization of extraction technology of total saponins from Trillium tschonoskii Rhizome by response surface method
10.3760/cma.j.cn115398-20201124-00205
- VernacularTitle:星点设计-效应面法优选头顶一颗珠总皂苷提取工艺研究
- Author:
Liying HE
1
;
Jing WANG
;
Yifan FENG
;
Jie CHEN
;
Hui ZHAO
;
Ping YU
;
Haiyan ZOU
Author Information
1. 首都医科大学中医药学院 中医络病研究北京市重点实验室，北京 100069
- Keywords:
Quality control;
Trillium tschonoskii rhizome;
Central composite design;
Response surface method;
Extraction process
- From:
International Journal of Traditional Chinese Medicine
2022;44(1):56-60
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the ethanol reflux extraction process of total saponins in total saponins of Trillium tschonoskii Rhizome. Methods:On the basis of single factor tests, making the total extraction rate of three main compounds [paris saponin Ⅵ, paris saponin Ⅶ and pennogenin-3- O-α-L-rhamnopyranosyl-(1→4)-[O-α-L-rhamnopyranosyl (1→2)]- O-β-D-glucopyranoside (PRRG)] as the indicator, the optimal extraction parameter was selected with the main influencing factors: the ethanol concentration, solid-liquid ratio, and extraction time by the central composite design-response surface method.Results:The optimal extraction parameter for the ethanol extract of total saponins of Trillium tschonoskii Rhizome was as follows: ethanol concentration 69%, extraction time 1.9 h, and solid liquid ratio 1∶9.7. The binomial fitting complex correlation coefficient r = 0.966 1, and the deviation between the extracted predicted value and the actual value is 4.68%. Conclusion:The central composite design-response surface method is simple and reliable for the optimization of extraction process of total saponins of Trillium tschonoskii Rhizome.
