Simultaneous determination of 10 components in Guanxinshutong capsules by quantitative analysis of multi-components by single marker
10.16438/j.0513-4870.2022-0123
- VernacularTitle:一测多评法同时测定冠心舒通胶囊中10个成分的含量
- Author:
Kai-hua LONG
1
;
Feng LIU
2
;
Hong ZHANG
1
;
Xia DU
1
;
Chun-liu WANG
1
;
Yang LIU
1
;
Dong-hua YANG
3
;
Ye LI
1
Author Information
1. Shaanxi Academy of Traditional Chinese Medicine, Xi'an 710003, China
2. Shaanxi Buchang Pharmaceutical Limited Company, Xi'an 710075, China; Shaanxi Institute of International Trade & Commerce, Xianyang 712046, China; Collaborative Innovation Center of Green Manufacturing Technology for Traditional Chinese Medicine in Shaanxi Province, Xi'an 710075, China
3. Qinghai Hospital of Traditional Chinese Medicine, Xining 810000, China
- Publication Type:Research Article
- Keywords:
Guanxinshutong capsule;
quantitative analysis of multi-components by single marker;
relative correction factor;
protocatechuic acid;
content determination
- From:
Acta Pharmaceutica Sinica
2022;57(6):1880-1886
- CountryChina
- Language:Chinese
-
Abstract:
This study establishes a quantitative analysis of multi-components by single marker (QAMS) method for the simultaneous determination of gallic acid, sodium danshensu, protocatechuic acid, protocatechuic aldehyde, vanillin, rosmarinic acid, salvianolic acid B, eugenol, cryptotanshinone and tanshinone IIA in Guanxinshutong capsules (Bambusae Concretio Silicea, Salvia miltiorrhiza, clove, borneol, Bambusae Concretio Silicea) by HPLC. Sample was loaded onto an Agilent C18 (ZORBAX Extend-RP C18, 250 mm × 4.6 mm, 5 µm) column and eluted with methanol-0.4% aqueous formic acid solution as a flow phase gradient, flow speed 1.0 mL·min-1, detection wavelength 280 nm, column temperature 35 ℃ and sample intake of 5 µL. Using protocatechuic acid as the internal reference, a relative correction factor was calculated and the durability was investigated, and the content of 10 components was calculated by QAMS and external standard method (ESM). The results show that the specificity, linear relationship, precision, repeatability, and stability of the 10 components were good. The average recovery was 98.20%-103.47% and RSD was 1.26%-2.84%. The relative positive factors and contents of the other nine components were calculated as gallic acid (0.759, 227.381), sodium tanshinol (3.630, 3.283), protocatechualdehyde (0.185, 0.150), vanillin (0.532, 65.213), rosmarinic acid (4.240, 1.035), salvianolic acid B (3.245, 18.204), eugenol (1.729, 9.265), cryptotanshinone (0.691, 1.449), and tanshinone ⅡA (0.702, 1.939). The results of QAMS were consistent with ESM analysis, and the relative error was between -3% and 3%. This method is stable and reliable, and can be used for the determination of 10 components in Guanxinshutong capsules.
