GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum.
10.4142/jvs.2013.14.3.241
- Author:
Hun Su SEO
1
;
Kyoung Hee KIM
;
Dae Yong KIM
;
Bong Kyun PARK
;
Nam Shik SHIN
;
Jae Hoon KIM
;
Heejeong YOUN
Author Information
1. Pfizer Animal Health, Seoul 135-784, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
GC/MS;
HPLC;
Neospora caninum;
Sophora flavescens;
Torilis japonica
- MeSH:
Apiaceae/*chemistry;
Chromatography, High Pressure Liquid;
Coccidiostats/*chemistry;
Fruit/chemistry;
Gas Chromatography-Mass Spectrometry;
Neospora/*drug effects/growth & development;
Plant Extracts/*chemistry;
Plant Roots/chemistry;
Sophora/*chemistry
- From:Journal of Veterinary Science
2013;14(3):241-248
- CountryRepublic of Korea
- Language:English
-
Abstract:
We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-beta-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.
