Vitamin D3 up-regulated protein 1 controls the priming phase of liver regeneration.
10.4142/jvs.2013.14.3.257
- Author:
Hyo Jung KWON
1
;
Sung Kuk HONG
;
Won Kee YOON
;
Ki Hoan NAM
;
In Pyo CHOI
;
Dae Yong KIM
;
Hyoung Chin KIM
;
Young Suk WON
Author Information
1. Biomedical Mouse Resource Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk 363-883, Korea. yswon@kribb.re.kr
- Publication Type:Original Article
- Keywords:
c-Jun-N-terminal kinase;
NF-kappaB;
partial hepatectomy;
signal transducer and activator of transcription 3;
Vitamin D3 up-regulated protein 1
- MeSH:
Animals;
Blotting, Western;
Carrier Proteins/*genetics/metabolism;
Cell Proliferation;
*Gene Expression Regulation;
Hepatectomy;
Hepatocytes/*cytology/physiology;
JNK Mitogen-Activated Protein Kinases/genetics/metabolism;
Liver/*physiology;
Male;
Mice, Knockout;
NF-kappa B/genetics/metabolism;
Polymerase Chain Reaction;
*Regeneration;
STAT3 Transcription Factor/genetics/metabolism;
Thioredoxins/*genetics/metabolism
- From:Journal of Veterinary Science
2013;14(3):257-262
- CountryRepublic of Korea
- Language:English
-
Abstract:
Vitamin D3 up-regulated protein 1 (VDUP1) is a potent growth suppressor that inhibits tumor cell proliferation and cell cycle progression when overexpressed. In a previous study, we showed that VDUP1 knockout (KO) mice exhibited accelerated liver regeneration because such animals could effectively control the expression of cell cycle regulators that drive the G1-to-S phase progression. In the present study, we further investigated the role played by VDUP1 in initial priming of liver regeneration. To accomplish this, VDUP1 KO and wild-type (WT) mice were subjected to 70% partial hepatectomy (PH) and sacrificed at different times after surgery. The hepatic levels of TNF-alpha and IL-6 increased after PH, but there were no significant differences between VDUP1 KO and WT mice. Nuclear factor-kappaB (NF-kappaB), c-Jun-N-terminal kinase (JNK), and signal transducer and activator of transcription 3 (STAT-3) were activated much earlier and to a greater extent in VDUP1 KO mice after PH. A single injection of TNF-alpha or IL-6 caused rapid activation of JNK and STAT-3 expression in both mice, but the responses were stronger and more sustained in VDUP1 KO mice. In conclusion, our findings provide evidence that VDUP1 plays a role in initiation of liver regeneration.
