Expression Level and Correlation of miR-211, miR-155, C-myc in Acute T Lymphocytic Leukemia.
10.19746/j.cnki.issn.1009-2137.2022.02.017
- Author:
Yang ZHENG
1
;
Liang CHEN
2
;
Shu-Xia GUO
3
;
Yin-Sen SONG
4
,
5
Author Information
1. Department of Translational Medicine Research Center, People's Hospital of Zhengzhou, Zhengzhou 450000, Henan Province, China.
2. Department of Laboratory, People's Hospital of Zhengzhou, Zhengzhou 450000, Henan Province, China.
3. Department of Blood Specialty, People's Hospital of Zhengzhou, Zhengzhou 450000, Henan Province, China.
4. Department of Translational Medicine Research Center, People's Hospital of Zhengzhou, Zhengzhou 450000, Henan Province, China,E-mail: df13607670608@
5. com.
- Publication Type:Journal Article
- Keywords:
C-myc;
acute T lymphocytic leukemia;
correlation;
miR-155;
miR-211
- MeSH:
Humans;
Leukocytes, Mononuclear;
MicroRNAs/genetics*;
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/genetics*;
Prognosis;
Proto-Oncogene Proteins c-myc/genetics*;
RNA, Messenger
- From:
Journal of Experimental Hematology
2022;30(2):425-429
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression and correlation of miR-211, miR-155, and C-myc in acute T lymphocytic leukemia (T-ALL), aiming to provide evidence for the diagnosis and treatment.
METHODS:A total of 96 T-ALL patients who were diagnosed and treated in People's Hospital of Zhengzhou from June 2014 to June 2017 were selected, and 69 healthy volunteers who had a physical examination were selected as control group in the same period. Real-time fluorescent quantitative PCR (RT-PCR) was used to determine the expression levels of miR-211, miR-155, and C-myc in peripheral blood mononuclear cells in each group. Kaplan-Meier was used to analyze the survival of T-ALL patients and correlation of miR-211, miR-155, and C-myc with prognosis. Pearson correlation analysis was used to evaluate the correlation of miR-211, miR-155, and C-myc with disease risk.
RESULTS:The expression levels of miR-211 mRNA, miR-155 mRNA, and C-myc mRNA in T-ALL group were higher than those in the control group (P<0.01), those in non-remission group were higher than those in remission group (P<0.01), and those in high-risk group were also higher than those in low-risk group and intermediate-risk group (P<0.01). The survival time of T-ALL patients with low miR-211 expression was longer than that with high miR-211 expression (P<0.01), that with low miR-155 expression was longer than that with high miR-155 expression (P<0.01), and that with low C-myc expression was also longer than that with high C-myc expression (P<0.01). The high expression of miR-211, miR-155, and C-myc was linearly positively correlated with high risk of disease (r=0.749, 0.781, 0.804).
CONCLUSION:The expressions of miR-211, miR-155, and C-myc are up-regulated in T-ALL patients, closely related to prognosis, and linearly positively correlated with disease risk.
