Establishment of An Animal Model of Acute B Lymphoblastic Leukemia with Minimal Residual Disease.
10.19746/j.cnki.issn.1009-2137.2022.02.010
- Author:
Xiao-Fu JIN
1
;
Ling-Zhen WANG
1
;
Ke LEI
2
;
Zeng-Yi DONG
2
;
Ren ZHONG
1
;
Han ZHAO
3
;
Li-Rong SUN
4
Author Information
1. Department of Pediatric Hematology, The Affiliated Hospital of Qingdao Univerisity, Qingdao 266000, Shandong Province, China.
2. Department of Laboratory Medicine, The Affiliated Hospital of Qingdao Univerisity, Qingdao 266000, Shandong Province, China.
3. Department of Pathology, The Affiliated Hospital of Qingdao Univerisity, Qingdao 266000, Shandong Province, China.
4. Department of Pediatric Hematology, The Affiliated Hospital of Qingdao Univerisity, Qingdao 266000, Shandong Province, China,E-mail:sunlr@vip.sina.com.
- Publication Type:Journal Article
- Keywords:
BALA/c-nu mouse;
acute B lymphoblastic leukemia;
minimal residual disease;
subcutaneous into tumor
- MeSH:
Animals;
Body Weight;
Disease Models, Animal;
Humans;
Mice;
Mice, Nude;
Neoplasm Transplantation;
Neoplasm, Residual;
Precursor Cell Lymphoblastic Leukemia-Lymphoma
- From:
Journal of Experimental Hematology
2022;30(2):381-385
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an animal model of acute B lymphoblastic leukemia (B-ALL) with minimal residual disease.
METHODS:The transplanted tumor was formed by subcutaneous injection of 2×107 Nalm-6 cells, and the body weight, activity status and tumor formation status of nude mice were observed. Peripheral blood, bone marrow, liver and spleen and other tissues of nude mice were taken for pathological examination to understand whether the success of subcutaneous modeling was accompanied by systemic metastasis.
RESULTS:There were 2×107 Nalm-6 cells injected subcutaneously in nude mice, (11.0±2.5) days later, the tumors of (3-4) × (3-4) mm were observed, the body weight of the nude mice was reduced and activity showed no limited. Infiltration of tumor cells in liver, spleen and bone marrow were observed in pathological sections.
CONCLUSION:The animal model of subcutaneous tumor of B-ALL was successfully established in nude mice.
