Establishment of a Mouse Model of Acquired Aplastic Anemia Mediated by Cyclophosphamide Combined with Cyclosporine.
10.19746/j.cnki.issn.1009-2137.2022.01.037
- Author:
Jian-Fei SHI
1
;
Dan-Li JIAO
1
;
Chen ZHAO
1
;
Li QI
2
,
3
;
Wei-Jiang LI
3
,
4
Author Information
1. College of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditonal Chinese Medicine, Shanghai 201203, China.
2. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China,E-mail: 13917912810@
3. com.
4. College of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditonal Chinese Medicine, Shanghai 201203, China,E-mail: keepbalance1972@
- Publication Type:Journal Article
- Keywords:
acquired aplastic anemia;
cyclophosphamide;
cyclosporine;
mouse model
- MeSH:
Anemia, Aplastic;
Animals;
Bone Marrow;
Cyclophosphamide;
Cyclosporine;
Female;
Mice;
Mice, Inbred BALB C
- From:
Journal of Experimental Hematology
2022;30(1):222-226
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a stable mouse model of acquired aplastic anemia.
METHODS:Female BALB/C mice aged 6 months were intraperitoneally injected with cyclophosphamide and cyclosporine for 14 days. The number of peripheral blood cells, the concentration of hemoglobin, the number of bone marrow nucleated cells, bone marrow smear, bone marrow pathological sections and other indexes were observed.
RESULTS:In BALB/C mice injected intraperitoneally with cyclophosphamide and cyclosporine, the number of peripheral blood cells and the concentration of hemoglobin were significantly decreased, especially the white blood cells and platelets. Bone marrow smear showed a significant decrease in the number of nucleated cells and bone marrow hyperplasia. Bone marrow pathology showed decreased hematopoietic cells and increased non-hematopoietic cells such as adipocytes.
CONCLUSION:The mouse model with intraperitoneal injection of cyclophosphamide and cyclosporine can meet the diagnostic criteria of acquired aplastic anemia, which can be used as a mouse model for the study of the pathogenesis and treatment of acquired aplastic anemia.
