The Expression and Clinical Correlation of the CD68 Factor in Acute Myeloid Leukemia Patients.
10.19746/j.cnki.issn.1009-2137.2022.01.013
- Author:
Zheng-Hua LIU
1
;
Ping-Ping WANG
1
;
Xiao-Jing YAN
2
Author Information
1. Department of Hematology, The First Affiliated Hospital of China Medical University， Shenyang 110001, Liaoning Province, China.
2. Department of Hematology, The First Affiliated Hospital of China Medical University， Shenyang 110001, Liaoning Province, China,E-mail:yanxiaojing_pp@hotmail.com.
- Publication Type:Journal Article
- Keywords:
CD68;
acute myeloid leukemia;
tumor-associated macrophages
- MeSH:
Bone Marrow;
Flow Cytometry;
Humans;
Leukemia, Myeloid, Acute;
Leukocytes;
Prognosis;
Remission Induction
- From:
Journal of Experimental Hematology
2022;30(1):77-83
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the expression and clinical characteristics of CD68 in bone marrow and peripheral blood of patients with acute myeloid leukemia (AML).
METHODS:The expression of CD68 in bone marrow blast cells was detected by four-color flow cytometry in 50 newly diagnosed AML patients and 23 controls. The expression of CD68 in peripheral blood of 85 newly diagnosed AML patients, 29 remission AML patients and 24 controls was detected by ELISA. The correlation between the expression rate of non-M3 AML bone marrow CD68, peripheral blood CD68 concentration and white blood cell count and other clinical data was compared respectively.
RESULTS:The median CD68 expression rate in myeloid leukemia cells of non-M3 AML patients was 19.7%, significantly higher than control (0.2%) (P<0.001). The median concentration of non-M3 CD68 in peripheral blood was 67.97 pg/ml, significantly higher than in control (29.94 pg/ml)（P<0.01）. There was no statistically significant difference in the plasma CD68 concentration of the peripheral blood between the newly diagnosed (45.72 pg/ml) and the remission stage (55.12 pg/ml) of non-M3 AML patients by paired analysis (P>0.05). The results showed that the higher the expression rate of CD68 in bone marrow, the higher the count of white blood cells in peripheral blood, and the lower the count of hemoglobin and platelet in peripheral blood. The higher the plasma concentration of CD68 in peripheral blood, the higher the white blood cell count and the lower the complete remission rate.
CONCLUSION:The expression of CD68 both in bone marrow and peripheral blood of patients with non-M3 AML is higher than that of control group. Patients with high expression of CD68 show a low rate of complete remission, suggesting that the expression level of CD68 is correlated with treatment response.
