Identification of chemical constituents in Zhachong Shisanwei Pills by UPLC-Q-TOF-MS/MS.
10.19540/j.cnki.cjcmm.20211215.301
- Author:
Shi-Qi DONG
1
;
Lu ZHAO
2
;
Dong-Xu ZHANG
2
;
Fan-Long YANG
1
;
Ai-Jie ZHANG
1
;
Juan-Lan XIAO
2
;
Hui-Rong FAN
1
Author Information
1. Key Laboratory of Radio-pharmacokinetics of Innovative Drug, Chinese Academy of Medical Sciences, Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College Tianjin 300192, China.
2. Tianjin University of Traditional Chinese Medicine Tianjin 301617, China.
- Publication Type:Journal Article
- Keywords:
UPLC-Q-TOF-MS/MS;
Zhachong Shisanwei Pills;
chemical component analysis;
pharmacodynamic material basis;
quality evaluation
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Drugs, Chinese Herbal/chemistry*;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2022;47(6):1546-1557
- CountryChina
- Language:Chinese
-
Abstract:
Zhachong Shisanwei Pills, composed of 13 Chinese medicinal materials, are used for treating the diseases such as hemiplegia, pain of muscles and bones, rheumatism, and joint pain. The chemical composition and pharmacodynamics of Zhachong Shisanwei Pills have not been reported. Ultra-performance liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS) was employed to quickly identify the chemical components of Zhachong Shisanwei Pills, which was performed with Shim-pack GIST C_(18) column(4.6 mm×150 mm, 5 μm). The gradient elution was conducted with methanol-0.05% acetic acid as the mobile phase. Electrospray ionization mass spectrometry(ESI-MS) was carried out in both positive and negative ion modes. The compounds were identidied based on accurate relative molecular weight, fragment ion species, and the MS data of reference substances and in literature. In conclusion, a total of 98 compounds were identified, including 19 organic acids, 36 flavonoids, 13 volatile oils, 8 tannins, 5 2-(2-phenylethyl)chromones, 5 amino acids, 3 sesquiterpenoids, 3 alkaloids, and 2 other compounds. This study characte-rized the chemical components of Zhachong Shisanwei Pills rapidly for the first time, laying a foundation for further research on the pharmacodynamic material basis and quality evaluation.
