Study on serum pharmacodynamic material basis of Gegen Qinlian Decoction in treatment of ulcerative colitis based on UHPLC-Q-Orbitrap-MS.
10.19540/j.cnki.cjcmm.20210928.201
- Author:
Tong LI
1
;
Yu LI
2
;
Cai-Juan ZHANG
1
;
Jun YANG
2
;
Lin-Ying ZHONG
1
;
Wen-Juan XU
1
;
Ling DONG
1
Author Information
1. School of Life Sciences, Beijing University of Chinese Medicine Beijing 102488, China.
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
- Publication Type:Journal Article
- Keywords:
Gegen Qinlian Decoction;
UHPLC-Q-Orbitrap-MS;
pharmacodynamic material basis;
ulcerative colitis
- MeSH:
Alkaloids;
Animals;
Chromatography, High Pressure Liquid/methods*;
Colitis, Ulcerative/drug therapy*;
Drugs, Chinese Herbal;
Mice;
Tandem Mass Spectrometry/methods*
- From:
China Journal of Chinese Materia Medica
2022;47(4):1073-1084
- CountryChina
- Language:Chinese
-
Abstract:
This study established a mouse model of ulcerative colitis and explored the serum transitional components of Gegen Qinlian Decoction by UHPLC-Q-Orbitrap-MS. Based on the exact relative molecular weight and MS/MS spectrum, 55 prototype components and 59 metabolites were identified from the model group, while 18 prototype components and 35 metabolites from the control group. The prototype components in serum were mainly flavonoids and the characteristic components of the model group were alkaloids. Glucuronidation, sulfonation, and glycosylation have been confirmed to be the main metabolic types in vivo. The results of comparative analysis of differences indicated that puerarin, baicalin, wogonoside, wogonin, chrysin, oroxylin A, berberine, berberrubine, and palmatine were the characteristic components in model state, which at the same time, were confirmed by pharmacological studies to be the serum pharmacodynamic material basis of Gegen Qinlian Decoction in the treatment of ulcerative colitis. This study has provided reference for explaining the metabolic transformation pattern and mechanism of action of Gegen Qinlian Decoction in vivo.
