Cloning and tissue expression analysis of the LepROT gene of Rana dybowskii.
- Author:
Tong WU
1
;
Tingting LIU
1
;
Yiming LIU
1
;
Xueying ZHAO
1
;
Yufen LIU
1
;
Peng LIU
1
;
Wenge ZHAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Aeromonas hydrophila; LepROT; NF-κB signaling pathway; Rana dybowskii; lipopolysaccharides
- MeSH: Animals; Cloning, Molecular; DNA, Complementary; Gene Expression Profiling; Gene Expression Regulation; Mammals/metabolism*; NF-kappa B/genetics*; Phylogeny; RNA, Messenger/genetics*; Ranidae/genetics*
- From: Chinese Journal of Biotechnology 2022;38(5):1859-1873
- CountryChina
- Language:Chinese
- Abstract: Leptin receptor overlapping transcript (LepROT) plays multiple roles in the regulation of immune systems. However, very little information is available about the anti-infectious mechanisms of amphibians LepROT. In this study, the cDNA sequence of the Rana dybowskii LepROT gene was determined by using RT-PCR and bioinformatics analysis. Then, the Aeromonas hydrophila (Ah) and lipopolysaccharides (LPS) infected models of R. dybowskii was constructed to obtain histopathological characteristics. Constitutive expression of LepROT mRNA and NF-κB signaling pathway were detected by real-time quantitative PCR. The full-length cDNA of LepROT gene was 396 bp and encoded 131 amino acids. Amino acid sequence analysis revealed LepROT shares 93.74% and 86.39% identity with homologues from other amphibians and mammals respectively, and the LepROT gene was quite conserved among different species. After infection, the relative expression levels of LepROT, NF-κB, IKKα and IKKβ mRNA were all significantly upregulated (P < 0.01), but showed a diverse temporal pattern of up-regulation in different tissues. Therefore, it was proposed that the LepROT gene of R. dybowskii might activate the NF-κB signaling pathway to exert anti-infectious effects, thus providing evidence for further extending the biological function of LepROT.
