Isolation and Characterization of Extracellular Vesicle from Mesenchymal Stem Cells of the Epidural Fat of the Spine
- Author:
Soo-Eun SUNG
1
;
Min-Soo SEO
;
Kyung-Ku KANG
;
Joo-Hee CHOI
;
Si-Joon LEE
;
Ju-Hyeon LIM
;
Seung Yun YANG
;
Seul-Ki KIM
;
Gun Woo LEE
Author Information
- Publication Type:Basic Study
- From:Asian Spine Journal 2022;16(2):153-161
- CountryRepublic of Korea
- Language:English
-
Abstract:
Methods:In this study, we isolated the exosomes using the tangential flow filtration (TFF) system with exosome-depleted fetal bovine serum and performed the characterization tests via western blotting, reverse transcription–polymerase chain reaction, nanoparticle tracking analysis (NTA), and transmission electron microscopy.
Results:In transmission electron microscopy, the exosome had a diameter of approximately 100–200 nm and had a spherical shape, whereas in the NTA, the exosome had an average diameter of 142.8 nm with a concentration of 1.27×1010 particles/mL. The flow cytometry analysis showed the expression of CD63 and CD81. The western blotting analysis showed the positive markers.
Conclusions:These findings showed that isolating the exosomes via TFF resulted in high-quality EF-MSC exosome yield. Further studies with exosomes from EF-MSC are needed to evaluate the function and role of the EF tissue.
