Simultaneous determination of seven components in Piqin oral liquid by dual-wavelength HPLC
10.12206/j.issn.1006-0111.202008007
- VernacularTitle:双波长高效液相色谱法同时测定枇芩口服液中7种成分的含量
- Author:
Qingfen WANG
1
;
Xiaoling LIU
1
;
Yuru YANG
1
;
Jie LI
1
Author Information
1. Department of Pharmacy, No. 909 Hospital of Joint Logistics Support Force of the PLA/the Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China.
- Keywords:
Piqin oral liquid;
HPLC;
double-wavelength;
content determination
- From:
Journal of Pharmaceutical Practice
2022;40(2):161-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography(HPLC) method for simultaneous determination of the contents of 7 components of chlorogenic acid, caffeic acid, paeoniflorin, ammonium glycyrrhizinate, baicalin, baicalein, and wogonin in Piqin oral liquid. Methods A double-wavelength HPLC method was performed. The column was Agilent Zorbax SB-C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was 0.02% phosphoric acid aqueous solution (A)-acetonitrile (B) gradient elution; Flow rate: 1.0 ml/min; Column temperature: 35℃; Injection volume: 20 μl; Detection wavelength: 0-18.0 min, 325 nm (detect chlorogenic acid, caffeic acid); 18.0-65.0 min, 280 nm (detect paeoniflorin, baicalin, baicalein, ammonium glycyrrhizinate, wogonin). Results The chlorogenic acid, caffeic acid, paeoniflorin, ammonium glycyrrhizinate, baicalin, baicalein and wogonin were completely separated. Seven components have a good linear relationship between the peak area and concentration, with the recoveries between 96.41% and 99.70%. Conclusion This method is simple, accurate and reproducible, and can be used for the quality control of Piqin oral liquid.
