Expression of CRELD2 in mouse tissues
10.12206/j.issn.1006-0111.202111038
- VernacularTitle:CRELD2在小鼠组织中的表达水平研究
- Author:
Fuqiang CHANG
1
;
Shuna WANG
1
;
Dongsheng WANG
1
;
Chaoyu MIAO
1
Author Information
1. Department of Pharmacology, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
CRELD2;
tissue specificity;
secreted protein.
- From:
Journal of Pharmaceutical Practice
2022;40(2):125-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of CRELD2 at the gene and protein levels of mouse tissues, and to provide a reference for studying the biological function of CRELD2 in various tissues. Methods The expression level of CRELD2 in the liver, pancreas, stomach, and lung of C57BL/6J mice was determined by real-time PCR and Western Blot. Results RT-PCR and WB showed that CRELD2 was expressed in mouse liver, pancreas, stomach, and lung. The relative expression levels of CRELD2 from high to low were pancreas, stomach, liver, and lung at the gene level, and pancreas, liver, stomach, and lung at protein level respectively. The result suggested that the relative expression levels of the CRELD2 gene and protein in different tissues were not completely consistent, suggesting that it is related to transcriptional regulation. Conclusion CRELD2 is expressed in mouse liver, pancreas, stomach, and lung, and the relative expression levels of CRELD2 are not completely parallel at the gene and protein level.
