Incomplete concerted evolution of nrDNA ITS in Lycium
10.16438/j.0513-4870.2021-1303
- VernacularTitle:枸杞属植物nrDNA ITS致同进化不完全
- Author:
Jiao ZHANG
1
;
Ying-lan NIE
1
;
Wei GUO
2
;
Dan QIAN
1
;
Lu-qi HUANG
3
Author Information
1. Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. Shandong Provincial Maternal and Child Health Care Hospital, Shandong University, Jinan 250014, China
3. China Academy of Chinese Medical Sciences, Beijing 100700, China
- Publication Type:Research Article
- Keywords:
italic>Lycium;
nrDNA ITS;
polymorphism;
incomplete concerted evolution;
pseudogenes
- From:
Acta Pharmaceutica Sinica
2022;57(2):525-532
- CountryChina
- Language:Chinese
-
Abstract:
In this study, nrDNA ITS sequences of Lycium cultivars were sequenced and used to test the existence of incomplete concerted evolution and pseudogenes. Together with 44 ITS sequences retrieved from GenBank, the pattern of base substitutions, GC content, 5.8S conserved motifs, the minimum free energy of secondary structures, nucleotide diversity and phylogenetic relationship of the samples were analyzed. While 83 of the 144 sequences were identified as pseudogenes, the results suggested a high degree of polymorphism and putative pseudogenes in Lycium, suggesting an incomplete concerted evolution of the ITS region. ITS polymorphism and pseudogene of Lycium were systematically tested for the first time. This research provides a references for ITS sequence to be used in the study of Lycium germplasm resources and DNA barcode identification.
