Establishment of a rapid method for quality assessment of cardiotonic effect of Shengfupian
10.16438/j.0513-4870.2021-1058
- VernacularTitle:一种关联生附片强心作用的质量快速评价方法的建立
- Author:
Lu-meng CHEN
1
;
Ya-nan HE
1
;
Hui LIU
1
;
Peng TAN
2
;
Hai-feng YUAN
3
;
Hong-yan MA
1
;
Jun-zhi LIN
4
;
Ming YANG
5
;
Ming-yan JIA
1
;
Ding-kun ZHANG
1
Author Information
1. Pharmacy School, State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2. State Key Laboratory of Biological Evaluation of TCM Quality, National Administration of TCM, Academy of Chinese Medicine Sciences, Chengdu 610041, China
3. Sichuan Xingshengyuan Pharmaceutical Co., Ltd., Jiangyou 621700, China
4. TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China
5. State Key Laboratory of Innovation Drug and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
Aconiti Lateralis Radix Praeparata;
cardiotonic action;
rapid inspection method;
alizarin red S;
phenylboronic acid;
quality evaluation
- From:
Acta Pharmaceutica Sinica
2022;57(2):460-466
- CountryChina
- Language:Chinese
-
Abstract:
Based on the similar structure of adrenaline shared by higenamine (HI), salsolinol (SA) and coryneine (CO), a photochemical colorimetric sensor based on the displacement reaction of o-diphenol hydroxyl group and alizarin red S-phenylboric acid system was constructed to quickly distinguish and identify the cardiac strength of Shengfupian. The results show that the optimal condition of the sensor is: the molar ratio of alizarin red S (ARS) to phenylboric acid (PA) is 1∶3, reaction temperature is 0 ℃; The preparation method of the sample solution is optimized as follows: 2.5 g of Shengfupian powder was taken, 10 times the amount of methanol was added, and 300 W, 40 kHz ultrasound was carried out for 15 min; methodological studies showed that the method had good precision, repeatability and stability. The |△G| value (G is green, |△G| = |G after - G before|) of each sample was obtained by response values determination of 14 batches of Shengfupian. LC-MS/MS was used to determine the contents of three cardiac components in Shengfupian. It was found that the order of the total contents of cardiotonic components was basically consistent with |△G|. Then the correlation was analyzed, and the correlation coefficient R2 was as high as 0.87, which proved the scientificity and accuracy of this method. This study fills the methodological gap of rapid evaluation of the quality of Shengfupian, and provides the key technical support for the high quality and good price of Shengfupian in the market circulation and clinical application.
