Construction of a blood-brain barrier microfluidic chip model and evaluation of the permeability of active components in traditional Chinese medicine
10.16438/j.0513-4870.2021-1811
- VernacularTitle:血脑屏障微流控芯片模型的构建及其中药活性成分跨膜转运研究
- Author:
Yi-wei SHI
1
;
Ying CAI
2
;
Xiao-li HE
2
;
Zhan-ying HONG
3
;
Yi-feng CHAI
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China
2. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
3. School of Pharmacy, Naval Medical University, Shanghai 200433, China; School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
- Publication Type:Research Article
- Keywords:
blood brain barrier;
microfluidic chip;
active components in traditional Chinese medicine;
rug permeability;
apparent permeability coefficient
- From:
Acta Pharmaceutica Sinica
2022;57(3):802-808
- CountryChina
- Language:Chinese
-
Abstract:
A blood-brain barrier microfluidic chip platform for studying the permeability of active components in traditional Chinese medicine was developed. This model used primary human brain microvascular endothelial cells on a microfluidic chip consisting of two perpendicularly-crossing channels and a single layer porous polycarbonate membrane. The physiological shear stress in the human vasculature was also modeled in this device. Cell viability on the chip was monitored by cell staining and immunofluorescence staining. The cells spread well and the structure of an intercellular adhesion protein was satisfactory. The permeability of fluorescent tracers and three model drugs and the functional expression of P-glycoprotein (P-gp)on the blood-brain barrier were investigated. The results show that the apparent permeability coefficients (Papp) of the fluorescent tracers and three model drugs were consistent with those reported in the literature, and P-gp on the chip showed normal function, indicating that there was a complete structure and a functional BBB. The permeability of six active components of traditional Chinese medicine was investigated through this microfluidic chip and the drug concentration was determined by HPLC-MS/MS to obtain the Papp of each component. The Papp of corydaline was (4.51 ± 1.90)×10-7 cm·s-1, the Papp of tetrahydropalmatine was (9.10 ± 6.59)×10-7 cm·s-1, and the Papp of imperatorin was (9.38 ± 2.53)×10-7 cm·s-1; the concentration of isoimperatorin, baicalin and chlorogenic acid was below the limit of quantification, which suggested that isoimperatorin, baicalin and chlorogenic acid have poor permeability in this BBB chip. This blood-brain barrier microfluidic platform possesses a complete barrier function and near-physiological conditions and could be a valuable in vitro tool for drug permeability evaluation.
