CRISPR-Cas9: A method for establishing rat models of drug metabolism and pharmacokinetics.
10.1016/j.apsb.2021.01.007
- Author:
Jian LU
1
;
Jie LIU
1
;
Yuanqing GUO
1
;
Yuanjin ZHANG
1
;
Yeye XU
1
;
Xin WANG
1
Author Information
1. Changning Maternity and Infant Health Hospital, East China Normal University, Shanghai 200051, China.
- Publication Type:Review
- Keywords:
AAV, adeno-associated virus;
ADMET, absorption, distribution, metabolism, excretion and toxicity;
Animal model;
BSEP, bile salt export pump;
CRISPR-Cas, clustered regularly interspaced short palindromic repeats-CRISPR-associated;
CRISPR-Cas9;
DDI, drug–drug interaction;
DMPK, drug metabolism and pharmacokinetics;
DSB, double-strand break;
Drug metabolism;
Gene editing;
HBV, hepatitis B virus;
HDR, homology directed repair;
HIV, human immunodeficiency virus;
HPV, human papillomaviruses;
KO, knockout;
NCBI, National Center for Biotechnology Information;
NHEJ, non-homologous end joining;
OATP1B, organic anion transporting polypeptides 1B;
OTS, off-target site;
PAM, protospacer-associated motif;
Pharmacokinetics;
RNP, ribonucleoprotein;
SD, Sprague–Dawley;
SREBP-2, sterol regulatory element-binding protein 2;
T7E I, T7 endonuclease I;
TALE, transcriptional activator-like effector;
TALEN, transcriptional activators like effector nucleases;
WT, wild-type;
ZFN, zinc finger nucleases;
crRNAs, CRISPR RNAs;
pre-crRNA, pre-CRISPR RNA;
sgRNA, single guide RNA;
tracRNA, trans-activating crRNA
- From:
Acta Pharmaceutica Sinica B
2021;11(10):2973-2982
- CountryChina
- Language:English
-
Abstract:
The 2020 Nobel Prize in Chemistry recognized CRISPR-Cas9, a super-selective and precise gene editing tool. CRISPR-Cas9 has an obvious advantage in editing multiple genes in the same cell, and presents great potential in disease treatment and animal model construction. In recent years, CRISPR-Cas9 has been used to establish a series of rat models of drug metabolism and pharmacokinetics (DMPK), such as
