Construction of acetyl-CoA and DBAT hybrid metabolic pathway for acetylation of 10-deacetylbaccatin III to baccatin III.
10.1016/j.apsb.2021.03.029
- Author:
Hao WANG
1
;
Bo-Yong ZHANG
1
;
Ting GONG
1
;
Tian-Jiao CHEN
1
;
Jing-Jing CHEN
1
;
Jin-Ling YANG
1
;
Ping ZHU
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, NHC Key Laboratory of Biosynthesis of Natural Products, CAMS Key Laboratory of Enzyme and Biocatalysis of Natural Drugs, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- Keywords:
10-Deacetylbaccatin III-10-β-O-acetyltransferase;
ACS, acetyl-CoA synthetase;
Bioconversion of 10-deacetylbaccatin III to baccatin III;
Biosynthetic pathway of acetyl-CoA;
DBAT, 10-deacetylbaccatin III-10-β-O-acetyltransferase;
Deacetylation of baccatin III;
E. coli, Escherichia coli;
GAPD, glyceraldehyde-3-phosphate dehydrogenase;
High-cell-density fermentation;
Microbial cell factory;
PANK, pantothenate kinase;
PDH, pyruvate dehydrogenase complex;
PGK, 1,3-bisphosphoglycerate kinase;
Se/acsL641P, acetyl-CoA synthetaseL641P of Salmonella enterica;
Taxol semi-synthesis;
aceE, pyruvate decarboxynase;
aceF, dihydrolipoamide transacetylase;
lpdA, lipoamide dehydrogenase
- From:
Acta Pharmaceutica Sinica B
2021;11(10):3322-3334
- CountryChina
- Language:English
-
Abstract:
10-Deacetylbaccatin III (10-DAB) C10 acetylation is an indispensable procedure for Taxol semi-synthesis, which often requires harsh conditions. 10-Deacetylbaccatin III-10-
