Effects of Artesunate Combined with Arsenious Acid on Proliferation and Apoptosis of Multiple Myeloma Cells via PI3K/AKT Signaling Pathway.
10.19746/j.cnki.issn.1009-2137.2021.06.022
- Author:
Gui-Fang HU
1
;
Ke DONG
1
;
Jing-Feng DONG
1
;
Yun WANG
1
;
Wu GAO
2
Author Information
1. Department of Hematology, Putuo Hospital Shanghai University of Traditional Chinese Medicine, Shanghai Putuo District Central Hospital, Shanghai 200062, China.
2. Department of Hematology, Putuo Hospital Shanghai University of Traditional Chinese Medicine, Shanghai Putuo District Central Hospital, Shanghai 200062, China E-mail: xfgw212212@sina.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Artesunate;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Multiple Myeloma;
Phosphatidylinositol 3-Kinases/metabolism*;
Proto-Oncogene Proteins c-akt/metabolism*;
Signal Transduction;
Vascular Endothelial Growth Factor A
- From:
Journal of Experimental Hematology
2021;29(6):1819-1824
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of artesunate and arsenous acid and their combination on the proliferation and apoptosis of human multiple myeloma cells and their mechanism.
METHODS:Human multiple myeloma cell line RPMI 8226 cells were cultured and treated with 0, 1, 2, 4, 8 nmol/L arsenous acid and 0, 40, 80, 160, 320 μmol/L artesunate, respectively. The inhibition of cell growth was detected by CCK-8 assay. The apoptosis rate was detected by flow cytometry. QPCR was used to detect the mRNA expression of cell proliferation and apoptosis-related factors. The expression of cell proliferation, apoptosis-related factors and PI3K/AKT pathway protein were detected by Western blot.
RESULTS:CCK-8 assay showed that the growth of multiple myeloma cells was inhibited by arsenous acid and artesunate. The IC
CONCLUSION:Artesunate combined with arsenous acid inhibits proliferation and promotes apoptosis of tumor cells through PI3K/AKT signaling pathway, and is superior to the effect of two drugs alone.
