Analysis of the Differential Expression of circRNA in Acute Myeloid Leukemia by GEO Database.
10.19746/j.cnki.issn.1009-2137.2021.06.005
- Author:
Wei QIN
1
;
Si-Xuan QIAN
2
;
Xiao-Hui CAI
1
;
Xu-Zhang LU
1
;
Hong-Ying CHAO
1
Author Information
1. Department of Hematology, The Affiliated Changzhou NO.2 People's Hospital Nanjing Medical University, Changzhou 213000, Jiangsu Province, China.
2. Department of Hematology, Jiangsu Provincial People's Hospital (The First Affiliated Hospital of Nanjing Medical University), Nanjing 210029, Jiangsu Province, China,E-mail: qiansx@medmail.com.cn.
- Publication Type:Journal Article
- MeSH:
Gene Expression;
Humans;
Leukemia, Myeloid, Acute/genetics*;
MicroRNAs/genetics*;
Oligonucleotide Array Sequence Analysis;
RNA, Circular
- From:
Journal of Experimental Hematology
2021;29(6):1719-1726
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the difference expression of circular RNA (circRNA) in acute myeloid leukemia (AML) by using bioinformatics method.
METHODS:The microarray chip data of AML was searched and downloaded from the Gene Expression Omnibus (GEO) of the National Center for Bioinformatics (NCBI). The differences between AML samples and control samples were analyzed by R software. The interaction between deregulated circRNA, miRNA and mRNA were predicted by miranda software and miRTarBase software. The circRNA-miRNA-mRNA regulatory network was constructed by using the cytoHubba plugin based on the Cytoscape software.
RESULTS:A total of 203 differential expression of circRNAs were finally collected, including down-regulated 161 circRNAs and up-regulated 42 circRNAs. CircRNA/miRNA/mRNA interaction network was constructed through software prediction. hsa_circ_0001080, hsa_circ_0004511, hsa_circ_0054211, hsa_circ_0001944 may be positively regulated the gene expression in AML.
CONCLUSION:Abnormal expression of circRNA in AML may become a new target for AML treatment.
