Extract from Zanthoxylum piperitum Induces Apoptosis of AGS Gastric Cancer Cells Through Akt/MDM2/p53 Signaling Pathway.
10.1007/s11655-021-3486-8
- Author:
Ye Seul PARK
1
;
Gun He NAM
1
;
Kyung Jo JO
1
;
Hye Won KAWK
1
;
Sang Yung KIM
2
;
Young Min KIM
3
Author Information
1. Department of Biological science and Biotechnology, College of Life science and Nano Technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea.
2. Department of Food Science & Bio Technology, Shinansan University, Deahakro Danwon-gu, Ansan-City, Gyenggi-do, Korea.
3. Department of Biological science and Biotechnology, College of Life science and Nano Technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, Daejeon, 34054, South Korea. ym1938@naver.com.
- Publication Type:Journal Article
- Keywords:
AGS gastric cancer cells;
Akt/MDM2/p53 signaling pathway;
Zanthoxylum piperitum;
active compounds;
apoptosis
- MeSH:
Animals;
Apoptosis;
Cell Line, Tumor;
Cell Proliferation;
Female;
Mice;
Mice, Inbred BALB C;
Plant Extracts/therapeutic use*;
Proto-Oncogene Proteins c-akt/metabolism*;
Signal Transduction;
Stomach Neoplasms/drug therapy*;
Tumor Suppressor Protein p53/metabolism*;
Zanthoxylum/metabolism*
- From:
Chinese journal of integrative medicine
2021;27(10):752-759
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To determine the effect of Zanthoxylum piperitum extracet (ZPE) on apoptosis and analyze anticancer substances in ZPE, changes in proteins related to apoptosis, and pathological changes in tumors in mouse.
METHODS:Fifteen 4-week-old female BALB/c nu/nu mice were divided into 3 groups depending on ZPE dose, with 5 in each group. AGS gastric carcinoma cells (1 × 10
RESULTS:High performance liquid chromatography (HPLC) analysis showed that ZPE contained organic sulfur compounds such as alliin and S-allylcysteine. MTT assay results revealed that ZPE (10-85 µ g/mL) could effectively inhibit the growth of AGS gastric cancer cells at higher concentrations (P<0.05, P<0.01). The annexin V & dead cell staining assay and cell cycle arrest assay confirmed a dose-dependent increase in the apoptosis rate and G
CONCLUSION:ZPE decreases AGS cell proliferation and induces apoptosis by inhibiting Akt and MDM2 expression.
