Study on bile acid metabolism of sleep-deprived mice regulated by Jiaotai Pills based on LC-MS.
10.19540/j.cnki.cjcmm.20210720.202
- Author:
Wei ZHENG
1
;
Xue WANG
2
;
Hong-Cui HAN
2
;
Zi-Qi JING
2
;
Wen-Wen ZHAO
2
;
Bao-Juan XUE
3
;
Yu-Jie ZHANG
2
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China College of Pharmacy, Heze University Heze 274015, China.
2. School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 102488, China.
3. Monitoring and Statistics Center, National Administration of Traditional Chinese Medicine Beijing 100027, China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
Jiaotai Pills;
UPLC-MS/MS;
bile acid;
sleep deprivation
- MeSH:
Animals;
Bile Acids and Salts;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Drugs, Chinese Herbal;
Mice;
Mice, Inbred ICR;
Reproducibility of Results;
Sleep;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2021;46(20):5382-5392
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the changes of bile acid(BA) levels in mice with sleep deprivation and the regulatory effect of Jiaotai Pills(JTP) on bile acid metabolism, this study established an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method for simultaneous determination of 23 BAs in mice. A total of 24 ICR mice were randomized into normal group, model group, and JTP group. Mice in the model group and JTP group were deprived of sleep at 20 h·d~(-1) by sleep deprivation apparatus for 8 consecutive days. Mice in the JTP group were given(ig, qd) JTP 3.3 g·kg~(-1) and those in the normal group and model group received(ig) the same volume of purified water. UPLC conditions are as follows: Waters ACQUITY UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm), gradient elution with the mobile phase of 0.1% formic acid in water-methanol. MS conditions are as below: negative-ion electrospray ionization, multiple reaction monitoring(MRM). Thereby, the content of 23 BAs in serum, liver, and ileum was determined and methodological investigation of the method was performed. The results showed that 23 BAs could be accurately determined within 15 min and the correlation coefficients were all higher than 0.99. The precision, accuracy, specificity, reproducibility, matrix effect, and recovery of BAs all met the requirement. The levels of BAs were significantly increased in the serum, liver, and ileum of sleep-deprived mice, but JTP can significantly reduce the levels. The UPLC-MS/MS method is simple, rapid, and accurate, which can be used for the determination of 23 BAs in biological samples, and JTP can adjust the elevated BA levels of sleep-deprived mice.
