Study on determination and quantity transfer of multi index components in Wenjing Decoction.
10.19540/j.cnki.cjcmm.20210623.301
- Author:
Ge-Lin WU
1
;
Fei TENG
2
;
Xiang-Wei LI
2
;
Bo-Nan LIU
2
;
Yu-Ping DU
1
;
Jing-Jing ZHU
2
;
Wei-Hong FENG
2
Author Information
1. Chinese Medicine Research Institute of Yangtze River Pharmaceutical Group Taizhou 225321, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Wenjing Decoction;
benchmark;
classic prescription;
quantity transfer
- MeSH:
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
Glycyrrhizic Acid;
Powders;
Quality Control
- From:
China Journal of Chinese Materia Medica
2021;46(19):5005-5014
- CountryChina
- Language:Chinese
-
Abstract:
Based on the textual research on literature, the key information of Wenjing Decoction were tested and identified, and 15 batches of lyophilized powder samples of Wenjing Decoction were prepared. The specific components, including paeoniflorin, glycyrrhizin, ginsenosides(Rg_1, Re and Rb_1), glycyrrhizic acid, and paeonol, were used as indexes to establish the HPLC method for quantitative evaluation, and the content ranges and transfer rates of these components were determined. The results showed that the contents of paeoniflorin, glycyrrhizin, ginsenosides Rg_1 + Re, ginsenoside Rb_1, glycyrrhizic acid, and paeonol in the 15 batches of samples were 0.62%-0.86%, 0.25%-0.76%, 0.14%-0.30%, 0.07%-0.21%, 0.63%-1.16%, and 0.09%-0.25%, respectively, and their transfer rates from the decoction pieces to the reference materials were 14.99%-19.42%, 28.11%-40.93%, 25.92%-61.88%, 25.03%-64.06%, 23.43%-35.53%, and 5.34%-10.44%, respectively. The consistency of the transfer rates between batches indicated that the preparation process was stable. It is suggested that the contents of paeoniflorin, glycyrrhizin, ginsenosides Rg_1 + Re, ginsenoside Rb_1, glycyrrhizic acid, and paeonol in Wenjing Decoction should not be less than 0.52%, 0.35%, 0.15%, 0.10%, 0.63%, and 0.12%, respectively. In this study, we determined the contents and analyzed the quantity transfer process of the index components in Wenjing Decoction, which can provide a basis for the follow-up development of Wenjing Decoction and the quality control of related preparations.
