Effects of Signaling Activation of Bone Morphogenetic Protein 2 on the Differentiation and Infiltration of Regulatory T Lymphocytes in Tumors.
10.3881/j.issn.1000-503X.13713
- Author:
Fei HUANG
1
;
Hai WANG
2
;
Yao-Qing CHEN
2
;
Gui WU
2
Author Information
1. Central Lab, the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China.
2. Department of Orthopedics,the First Affiliated Hospital of Fujian Medical University,Fuzhou 350005,China.
- Publication Type:Journal Article
- Keywords:
bone morphogenetic protein 2;
myeloid-derived suppressor cells;
regulatory T lymphocyte;
tumor
- MeSH:
Animals;
Bone Morphogenetic Protein 2;
Cell Differentiation;
Mice;
Myeloid-Derived Suppressor Cells;
Neoplasms;
T-Lymphocytes, Regulatory;
Transforming Growth Factor beta
- From:
Acta Academiae Medicinae Sinicae
2021;43(6):897-904
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine whether the signaling activation of bone morphogenetic protein 2(BMP2)can induce myeloid-derived suppressor cells(MDSC)to secret transforming growth factor β(TGF-β),further enhancing the differentiation and infiltration of regulatory T lymphocytes(Treg)into tumor tissue. Methods The BMP2-induced mRNA and protein expression of TGF-β in MDSC was detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA),respectively.The effect of BMP2-induced TGF-β secretion by MDSC on Treg differentiation was then determined by flow cytometry.Finally,we implanted the recombined human bone morphogenetic protein 2(rhBMP2)collagen gels into tumor-burdened mice to examine the role of BMP2 in Treg differentiation via MDSC-secreted TGF-β
