Long non-coding RNA potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 regulates the proliferation and osteogenic differentiation of human periodontal ligament stem cells by targeting miR-24-3p.

Ming PANG; Hong-Xia WEI; Xi CHEN

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Long non-coding RNA potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 regulates the proliferation and osteogenic differentiation of human periodontal ligament stem cells by targeting miR-24-3p.

Author: Ming PANG ¹ ; Hong-Xia WEI ¹ ; Xi CHEN ²
Author Information

1. Dept. of Stomatology, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545005, China.
2. Dept. of Stomatology, Beijing Rehabilitation Hospital of Capital Medical University, Beijing 100144, China.
Publication Type:Journal Article
Keywords: human periodontal ligament stem cells; miR-24-3p; osteogenic differentiation; potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1; proliferation
MeSH: Cell Differentiation; Cell Proliferation; Humans; MicroRNAs/genetics*; Osteogenesis; Periodontal Ligament/cytology*; Potassium; Potassium Channels, Voltage-Gated; RNA, Long Noncoding/genetics*; Stem Cells/cytology*
From: West China Journal of Stomatology 2021;39(5):547-554
CountryChina
Language:English
Abstract: OBJECTIVES:This study aims to explore the effect and molecular mechanism of long non-coding RNA (lncRNA) potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 (KCNQ1OT1) on proliferation and osteogenic differentiation in human periodontal ligament stem cells (hPDLSCs).
METHODS:The hPDLSCs of normal periodontal tissues were isolated and cultured. The mineralized solution induced the osteoblast differentiation of hPDLSCs. The down-regulation of lncRNA KCNQ1OT1, the overexpression of anti-miR-24-3p on the proliferation and the levels of osteocalcin (OCN), osteopontin (OPN) and alkaline phosphatase (ALP) of hPDLSCs were investigated. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the levels of lncRNA KCNQ1OT1, miR-24-3p, OCN, OPN, and ALP. Methyl thiazolyl tetrazolium (MTT) method was used to detect cell viability and activity. Cell proliferation was evaluated by MTT. Western blot was used to detect protein expression. The targeted relationship between lncRNA KCNQ1OT1 and miR-24-3p was detected by double-luciferase experiment.
RESULTS:The expression level of lncRNA KCNQ1OT1 increased, and that of miR-24-3p decreased during the osteogenesis of hPDLSCs (
CONCLUSIONS:Down-regulation of lncRNA KCNQ1OT1 inhibited the proliferation and osteogenic differentiation of hPDLSCs by targeting the up-regulated expression of miR-24-3p.