Effect of Mitomycin C, Dexamethasone, and Cyclosporine A 0.05% on the Proliferation of Human Corneal Keratocytes.
10.3341/jkos.2011.52.10.1215
- Author:
Jong Hoon SHIN
1
;
Soo Jin KIM
;
Ji Eun LEE
;
Jong Soo LEE
Author Information
1. Department of Ophthalmology, Pusan National University College of Medicine, Busan, Korea. jongsool@pusan.ac.kr
- Publication Type:Original Article ; In Vitro
- Keywords:
Cyclosporine;
Dexamethasone;
Keratocyte;
Mitomycin C
- MeSH:
Annexin A5;
Apoptosis;
Cell Proliferation;
Cells, Cultured;
Collagen Type I;
Corneal Keratocytes;
Corneal Opacity;
Cyclosporine;
Dexamethasone;
Flow Cytometry;
Humans;
L-Lactate Dehydrogenase;
Laminin;
Mitomycin
- From:Journal of the Korean Ophthalmological Society
2011;52(10):1215-1221
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the biologic effect of mitomycin C, dexamethasone and cyclosporine A 0.05% on cultured human keratocytes in vitro. METHODS: Human corneal keratocytes were exposed to a concentration of mitomycin C (0.05%), dexamethasone (0.05%) and cyclosporine A (0.05%) for a period of 3, 5, and 10 minutes. MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation and the concentration of type I procollagen COOH-terminal peptide (PIP) and laminin were measured. Cell damage was determined by using the lactate dehydrogenase (LDH) assay. Apoptotic response was evaluated utilizing flow cytometric analysis with Annexin V and propiodium iodide. RESULTS: The inhibitory effect of cellular proliferation and cytotoxicity in cultured human keratocytes showed a time-dependent response in all drugs. The production of PIP and laminin showed a time-dependent response in cultured cells. Apoptosis was observed in flow cytometry after being treated with mitomycin C, dexamethasone and cyclosporine A. Cyclosporin A resulted in less apoptosis of keratocytes than mitomycin C and dexamethasone. CONCLUSIONS: The apoptotic response of mitomycin C, dexamethasone and cyclosporine A is associated with the inhibitory effect of human corneal keratocyte proliferation. To decrease corneal opacity, mitomycin C and dexamethasone were more effective than cyclosporine A in the present study. Additionally, a high concentration of cyclosporine A greater than 0.05% is necessary to lower corneal opacity.
