Chemical derivatization in LC-MS/MS method for clinical detection
10.3760/cma.j.cn114452-20210508-00289
- VernacularTitle:液相色谱-串联质谱化学衍生技术在临床检验中的应用
- Author:
Shaoting WANG
1
;
Wen DAI
;
Yan LI
Author Information
1. 武汉大学人民医院检验医学中心，武汉 430060
- Keywords:
Liquid chromatography-tandem mass spectrometry;
Chemical derivatization;
Vitamins;
Steroids;
Neurotransmitters;
Therapeutic drug monitoring
- From:
Chinese Journal of Laboratory Medicine
2021;44(8):664-668
- CountryChina
- Language:Chinese
-
Abstract:
Low ionizability and poor chemical instability make some target analytes not suitable for direct LC-MS/MS analysis. To improve the detection performance, chemical derivation is frequently imported. However, such technology involves multiple disciplines such as laboratory medicine, organic chemistry and separation science. At present, it′s still a challenge for general application of chemical derivatization in routine clinical laboratories in our country. This article summarizes the key scientific connotation of chemical derivatization in the detection of vitamins, steroids, neurotransmitters and therapeutic drugs, and describes the derivatization approaches for development of chemical mass spectrometry in the future.
