Fucoidan attenuates 6-hydroxydopamine-induced neurotoxicity by exerting anti-oxidative and anti-apoptotic actions in SH-SY5Y cells.
10.14405/kjvr.2017.57.1.1
- Author:
Myung Hwan KIM
1
;
Hoon NAMGOONG
;
Bae Dong JUNG
;
Myung Sang KWON
;
Yeon Shik CHOI
;
Taekyun SHIN
;
Hyoung Chun KIM
;
Myung Bok WIE
Author Information
1. College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea. mbwie@kangwon.ac.kr
- Publication Type:Original Article
- Keywords:
apoptosis;
caspase-3/7;
fucoidan;
glutathione/glutathione disulfide ratio;
6-hydroxydopamine
- MeSH:
Apoptosis;
Cell Death;
Flow Cytometry;
Glutathione;
Lipid Peroxidation;
Membrane Potential, Mitochondrial;
Nervous System Diseases;
Neurons;
Oxidopamine;
Parkinson Disease;
Reactive Oxygen Species
- From:Korean Journal of Veterinary Research
2017;57(1):1-7
- CountryRepublic of Korea
- Language:English
-
Abstract:
Parkinson's disease (PD) is an irreversible neurological disorder with related locomotor dysfunction and is haracterized by the selective loss of nigral neurons. PD can be experimentally induced by 6-hydroxydopamine (6-OHDA). It has been reported that reactive oxygen species, which deplete endogenous glutathione (GSH) levels, may play important roles in the dopaminergic cell death characteristic of PD. Fucoidan, a sulfated algal polysaccharide, exhibits anti-inflammatory and anti-oxidant actions. In this study, we investigated whether fucoidan can protect against 6-OHDA-mediated cytotoxicity in SH-SY5Y cells. Cytotoxicity was evaluated by using MTT and LDH assays. Fucoidan alleviated cell damage evoked by 6-OHDA dose-dependently. Fucoidan reduced the number of apoptotic nuclei and the extent of annexin-V-associated apoptosis, as revealed by DAPI staining and flow cytometry. Elevation of lipid peroxidation and caspase-3/7 activities induced by 6-OHDA was attenuated by fucoidan, which also protected against cytotoxicity evoked by buthionine-sulfoximine-mediated GSH depletion. Reduction in the glutathione/glutathione disulfide ratio induced by 6-OHDA was reversed by fucoidan, which also inhibited 6-OHDA-induced disruption of mitochondrial membrane potential. The results indicate that fucoidan may have protective action against 6-OHDA-mediated neurotoxicity by modulating oxidative injury and apoptosis through GSH depletion.
